Establishment of quality evaluation methods for pieces and standard decoction of honey-fried Descurainiae Semen.
10.19540/j.cnki.cjcmm.20191230.301
- Author:
Xiu-Wen WANG
1
;
Zhao-Jie MA
2
;
Ru-Na JIN
3
;
Yun-Tao DAI
3
;
Shuo-Sheng ZHANG
1
Author Information
1. Shanxi University of Traditional Chinese Medicine Jinzhong 030619, China.
2. Shanxi University of Traditional Chinese Medicine Jinzhong 030619, China Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
3. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
- Publication Type:Journal Article
- Keywords:
fingerprint;
honey-fried Descurainiae Semen;
quality evaluation;
quercetin-3-O-β-D-glucose-7-O-β-D-gentiobioside;
standard decoction
- MeSH:
Brassicaceae/chemistry*;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal/standards*;
Glucosides/analysis*;
Honey;
Quality Control;
Quercetin/analogs & derivatives*
- From:
China Journal of Chinese Materia Medica
2020;45(4):884-889
- CountryChina
- Language:Chinese
-
Abstract:
To establish a content determination method for quality control of the pieces and standard decoction of honey-fried Descurainiae Semen. Standard decoction of honey-fried Descurainiae Semen was prepared with standardized process, and high performance liquid chromatography coupled with diode-array detector(HPLC-DAD) was used to detect its characteristic fingerprint and determine the content of quercetin-3-O-β-D-glucose-7-O-β-D-gentiobioside. In addition, the transfer rate, dry extract rate and pH value were calculated. The results showed that the established method had a high accuracy. The content of quercetin-3-O-β-D-glucose-7-O-β-D-gentiobioside in 13 batches of standard decoction was 0.03-0.12 mg·mL~(-1); the transfer rate was 13.4%-23.1%; the rate of extracts was 1.9%-5.5%, and the pH was between 5.4-5.9. The similarity coefficients were all greater than 0.85, indicating good homogeneity for the different batches of decoction. There were 7 common peaks in the characteristic chromatogram, one of which was quercetin-3-O-β-D-glucose-7-O-β-D-gentiobioside. In this paper, the established content determination and quality evaluation method for Descurainiae Semen pieces and decoction was simple, rapid and reproducible, providing reference for the quality control of honey-fried Descurainiae Semen pieces, standard decoction and its preparations.
