Transcriptome and quality evaluation of Sichuan Dao-di herbs Zanthoxylum bungeanum.
10.19540/j.cnki.cjcmm.20191226.102
- Author:
Hua HUA
1
;
Zhi-Xiang YAN
1
;
Wei-Wei TIAN
1
;
Li LIU
1
;
Hong-Bing SUN
1
;
Xian-Jian ZHOU
1
;
Liang-Chun YAN
1
;
Jun-Ning ZHAO
1
Author Information
1. Institute of Translational Pharmacology and Clinical Application, Sichuan Center for Translational Medicine of Traditional Chinese Medicine, Sichuan Engineering Technology Research Center of Genuine Regional Drug, Biological Assay Key Laboratory of State Administration of Traditional Chinese Medicine for Traditional Chinese Medicine Quality, Sichuan Provincial Key Laboratory of Quality Evaluation and New Drug Creation of Traditional Chinese Medicine, Laboratory for Rapid Detection of Toxicity of Traditional Chinese Medicine in Sichuan Provincial Administration of Chinese Medicine, Sichuan Academy of Chinese Medicine Sciences Chengdu 610041, China.
- Publication Type:Journal Article
- Keywords:
Sichuan Dao-di herbs;
Zanthoxylum bungeanum;
quality;
transcriptome
- MeSH:
China;
Fruit/chemistry*;
Gene Expression Profiling;
Plant Leaves/chemistry*;
Plants, Medicinal/chemistry*;
Secondary Metabolism;
Transcriptome;
Zanthoxylum/chemistry*
- From:
China Journal of Chinese Materia Medica
2020;45(4):732-738
- CountryChina
- Language:Chinese
-
Abstract:
"Huajiao" is dried ripe fruit peel of Zanthoxylum bungeanum or Z. schinifolium, is konwn as geoherbs, especially the "Dahongpao" cultivated in Hanyuan, Maoxian and Jiulong of Sichuan province. However, the genetic basis of Dao-di "Huajiao" is virtually unknown. The transcriptome of the fruit and leaf from Sichuan(Hanyuan, Jiulong, Lixian, Maoxian), Gansu(Wudu) province and Shaanxi(Fengxian) province was sequenced. Trinity de novo assembling resulted in a total of 177 616 unigenes. Through the KEGG, NR, SwissProt, Trembl, KOG/COG, GO, Pfam database comparision 106 644 annotated Unigene finally, 4 574 deferentially expressed genes were found in fruit between Sichuan and other provinces, including 3 740 up-regulated genes and 834 down-regulated genes. Among the up-regulated genes, 27 up-regulated genes were raleted to terpenoids, and 8 up-regulated genes were related to isoquinoline alkaloid bio-synthesis. Furthermore, it was also showed remarkable differences in groups which enrichment ratio of the diffe-rent expressed gene compared. The different expressed genes were annotated by the KEGG database into plant-pathogen interaction, plant hormone signal transduction and phenylpropanoid biosynthesis in fruit and leaf, but isoflavonoid bio-synthesis and betaine bio-synthesis were significantly different in fruit and leaf. The study laid a certain reference basis for comparison of quality and different expressed gene of Z. bungeanum from different groups.
