Effect of ginsenosides on serous metabonomic profiles in cerebral ischemia-reperfusion rats based on ~1H-NMR.
10.19540/j.cnki.cjcmm.20190619.505
- Author:
Dong-Min CAO
1
;
Qin-Xiao GUAN
1
;
Ya-Li LIU
1
;
Shu-Mei WANG
1
Author Information
1. School of Traditional Chinese Medicine, Guangdong Pharmaceutical University Guangzhou 510006, China Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica of State Administration of Traditional Chinese Medicine Guangzhou 510006, China Engineering Technology Research Center for Chinese Materia Medica Quality of Guangdong Province Guangzhou 510006, China.
- Publication Type:Journal Article
- Keywords:
ginsenosides;
metabonomics;
middle cerebral artery occlusion;
~1H-NMR
- MeSH:
3-Hydroxybutyric Acid;
Animals;
Brain Ischemia/metabolism*;
Creatine;
Ginsenosides/pharmacology*;
Hemolymph;
Metabolome;
Phosphocreatine;
Proton Magnetic Resonance Spectroscopy;
Random Allocation;
Rats;
Reperfusion Injury/metabolism*
- From:
China Journal of Chinese Materia Medica
2020;45(5):1142-1148
- CountryChina
- Language:Chinese
-
Abstract:
Serum metabonomic profiles of the model of focal cerebral ischemia reperfusion is established with the suture-occluded method by Longa to study the effect of ginsenosides. In this study, 48 rats were randomly divided into six groups: sham-operated group, pathological model group, positive drug group(6 mg·kg~(-1)·d~(-1)) and high, medium, low-dose ginsenosides groups(200, 100, 50 mg·kg~(-1)·d~(-1)). They are given intragastric administration respectively with same amount of 0.5% CMC-Na,nimodipine and ginsenoside for 5 days. At 2 h after the final administration, the model was established with the suture-occluded method, and free radical-scavenging activity changes of ginsenoside were observed by maillard reaction, and Longa was possible used as a renoprotective agent-occluded method. At the end of 24 h after the reperfusion, the hemolymph of rats in each group was collected, and the ~1H-NMR spectrum was collected after being treated by certain methods, and analyzed by principal component analysis(PCA). Compared with sham-operated group, pathological model group showed significant increases in the levels of lactate, glutamate, taurine, choline, glucose and methionine, but decreases in the levels of 3-hydroxybutyrate and phosphocreatine/creatine in serum. After treatment with ginsenosides, lipid, 3-hydroxybutyrate and phosphocreatine/creatine were increased in the serum of ginsenosides group rats, but with decreases in lactate and glutamate. The results showed that ginsenosides could regulate metabolic disorders in rats with focal cerebral ischemia reperfusion, and promote a recovery in the process of metabolism. It's helpful to promote the metabolic changes in rats with focal cerebral ischemia reperfusion via ~1H-NMR, and lay a foundation to develop ginsenosides as a new drug to treat ischemic cerebral paralysis.
