Study on non-flavonoids chemical constituents from Spatholobi Caulis.
10.19540/j.cnki.cjcmm.20200102.201
- Author:
Xiao-Yan LIU
1
;
Wei XU
1
;
Xiu-Wei YANG
1
;
Peng ZHANG
2
;
Wei ZHAO
2
;
Yun GONG
2
;
Ni-Fu LIU
2
Author Information
1. State Key Laboratory of Natural and Biomimetic Drugs, Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University Beijing 100191, China.
2. Zhuzhou Qianjin Pharmaceutical Co., Ltd. Zhuzhou 412000, China.
- Publication Type:Journal Article
- Keywords:
Spatholobi Caulis;
components;
non-flavonoids;
water extract
- MeSH:
Drugs, Chinese Herbal/analysis*;
Esters/analysis*;
Fabaceae/chemistry*;
Hydroxybenzoates/analysis*;
Mass Spectrometry;
Phytochemicals/analysis*
- From:
China Journal of Chinese Materia Medica
2020;45(5):1120-1127
- CountryChina
- Language:Chinese
-
Abstract:
To study the non-flavonoids chemical constituents in water extract of Spatholobi Caulis. Some purification and analysis techniques like silica gel, D101-macroporous adsorptive resins, and Sephadex LH-20 column chromatographies as well as reversed phase high-performance liquid chromatography were used to isolate and analyze the phenolic acid esters and other type compounds from Spatholobi Caulis integrally. The structures of these compounds were identified by spectroscopic techniques such as nuclear magnetic resonance and high resolution mass spectrometries. Twenty-seven compounds, including phenolic acid, coumarin, lignan, terpene, alkaloid, and steroid compounds, were isolated from ethyl acetate and n-butanol fractions in water extract of Spatholobi Caulis, and they were identified as β-sitosterol(1), feruli acid methyl ester(2), syringaresinol(3),(+)-medioresinol(4),(+)-epipinoresinol(5), p-acetylphenol(6), bolusanthin Ⅳ(7), evofolin B(8), salicylic acid(9), trans-p-hydroxy-cinnamic acid(10), abscisic acid(11), m-hydroxyphenol(12), C-veratroylglycol(13), p-hydroquinone(14), 8,9-dihydroxymegastigma-4,6-dien-3-one(15), p-hydroxybenzoic acid(16), 6,9-dihydroxymegastigma-4,7-dien-3-one(17), protocatechuic acid(18), protocatechuic acid methyl ester(19), 5,7-dihydroxycoumarin(20), isolariciresinol(21), nicotinic acid(22), daucosterol(23),(+)-pinoresinol(24), stigmasterol(25), allantoin(26) and koaburaside(27), respectively. Furthermore, compounds 2-15, 19-22, 24 and 26 were isolated from genus Spatholobus for the first time.
