Rapid quality detection system of Lonicerae Japonicae Flos formula granules.
10.19540/j.cnki.cjcmm.20200112.103
- Author:
Zi-Yuan CHEN
1
;
Li WANG
1
;
Chao JIANG
1
;
Yan JIN
1
;
Jun-Hui ZHOU
1
;
Tie-Gui NAN
1
;
Yuan YUAN
1
Author Information
1. National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
- Publication Type:Journal Article
- Keywords:
ELISA;
Lonicerae Japonicae Flos;
allele-specific PCR;
formula granule;
quality detection
- MeSH:
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal/analysis*;
Enzyme-Linked Immunosorbent Assay;
Lonicera/chemistry*;
Medicine, Chinese Traditional;
Polymerase Chain Reaction;
Quality Control
- From:
China Journal of Chinese Materia Medica
2020;45(5):1070-1075
- CountryChina
- Language:Chinese
-
Abstract:
The application of traditional Chinese medicine(TCM) formula granules in clinical practice is gradually extensive. However, TCM formula granules is still lacking rapid and simple quality control standards. In this study, allele-specific PCR and enzyme-linked immunoassay(ELISA) was used for rapid detection of the quality of Lonicerae Japonicae Flos formula granules. The authenticity of Lonicerae Japonicae Flos formula granules was identified by allele-specific PCR and index component was detected by ELISA. Thus, it lays a foundation for the establishment of rapid quality detection standard for Lonicerae Japonicae Flos formula granules, and also provides reference for other studies on the quality standard of traditional Chinese medicine formula granules.
