Expression characteristics of inflammatory and apoptosis factors and regulatory effect of anemoside B4 in mice with acute kidney injury.
10.19540/j.cnki.cjcmm.20190918.402
- Author:
Jun LI
1
;
Sha-Sha ZUO
1
;
Xiao-Xuan QIU
2
;
Xi XU
1
;
Ying-Ying LUO
1
;
Hong-Wei GAO
3
;
Yu-Lin FENG
4
;
Li-Jun DU
5
;
Qin GONG
1
Author Information
1. State Key Laboratory of Innovative Drugs and Efficient Energy-saving Pharmaceutical Equipment, Jiangxi University of Traditional Chinese Medicine Nanchang 330006, China.
2. Nanchang Ninth Hospital Nanchang 330006, China.
3. College of Pharmacy, Guangxi University of Chinese Medicine Nanning 530001, China.
4. State Key Laboratory of Innovative Drugs and Efficient Energy-saving Pharmaceutical Equipment, Jiangxi University of Traditional Chinese Medicine Nanchang 330006, China College of Pharmacy, Guangxi University of Chinese Medicine Nanning 530001, China.
5. State Key Laboratory of Innovative Drugs and Efficient Energy-saving Pharmaceutical Equipment, Jiangxi University of Traditional Chinese Medicine Nanchang 330006, China College of Pharmacy, Guangxi University of Chinese Medicine Nanning 530001, China School of Life Sciences, Tsinghua University Beijing 100084, China.
- Publication Type:Journal Article
- Keywords:
acute kidney injury;
anemoside B4;
apoptosis;
cisplatin;
inflammatory
- MeSH:
Acute Kidney Injury/drug therapy*;
Animals;
Apoptosis;
Apoptosis Regulatory Proteins;
Cytokines;
Inflammation;
Kidney;
Mice;
Saponins/therapeutic use*
- From:
China Journal of Chinese Materia Medica
2020;45(1):157-162
- CountryChina
- Language:Chinese
-
Abstract:
This paper was aimed to observe the effect of anemoside B4(hereinafter referred to as B4) on cisplatin-induced acute kidney injury in mice, and to investigate its possible mechanism in renal protection from inflammation and apoptosis aspects. Mice were divided into normal group, model group, dexamethasone positive group and B4 high, middle and low dose groups(5, 2.5, and 1.25 mg·kg~(-1 )doses). All the other mice groups except normal group were given with tail vein injection of cisplatin(15 mg·kg~(-1)) to induce acute kidney injury models. The drug administration was started on the day of modeling, and lasted for 4 days. After 1 hour of the last injection, orbital blood was collected. After the serum was separated, serum urea nitrogen(BUN), creatinine(Cre), total protein(TP), and albumin(ALB) were tested by using an automatic biochemical analyzer; the changes of kidney pathological morphology were observed by PAS staining; the protein expression levels of inflammatory factors including nucleotide binding oligomerization domain-like receptor(NLRP3), cysteinyl aspartate specific proteinase 1(caspase-1), interleukin-18(IL-18), interleukin-1β(IL-1β), tumor necrosis factor(TNF-α), and interleukin-6(IL-6) and apoptosis factors including p53, caspase-3, cleaved-caspase-3, Bcl-2 associated X protein(Bax), and B-cell lymphoma-2(Bcl-2) were analyzed by Western blot. The results showed that B4 significantly reduced the serum BUN and Cre contents, and alleviated pathological changes in renal tissues, such as the shedding and degeneration of renal tubular epithelial cells, tubulin tubule type. B4 significantly down-regulated the protein expressions of p53, Bax, cleaved-caspase-3 in the kidney and up-regulated the expression of Bcl-2/Bax. In model group, however, no significant up-regulation was observed in the protein expression levels of inflammatory cytokines(NLRP3, pro-caspase-1, IL-18, IL-1β, TNF-α, IL-6). The results suggested that B4 had a certain protective effect on cisplatin-induced acute kidney injury, and could activate p53 signaling pathway related apoptotic factors. B4 renal protective effect was mainly related to the regulation of p53 signaling pathway, while NLRP3 inflammasome and related inflammatory factors had no obvious response in this model.
