Analysis on transcriptionomic characteristics of Naoxintong Capsules in prevention of post-ischemic inflammation based on RNA-Seq technology.
10.19540/j.cnki.cjcmm.20190918.404
- Author:
Lei-Lei GONG
1
;
Hai-Yu XU
2
;
Lan WANG
2
;
Xiao-Jie YIN
2
;
Li LI
2
;
Wei WANG
3
;
Ri-Xin LIANG
2
;
Hong-Jun YANG
2
Author Information
1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China Pharmacy Department, Beijing Obstetrics and Gynecology Hospital, Capital Medical University Beijing 100026, China.
2. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
3. Department of Chinese Material Medica, Capital Medical University Beijing 100069, China.
- Publication Type:Journal Article
- Keywords:
Naoxintong Capsules;
RNA-Seq;
inflammation;
transcriptomics
- MeSH:
Animals;
Capsules;
Drugs, Chinese Herbal/therapeutic use*;
Inflammation/prevention & control*;
Ischemia/complications*;
Lipopolysaccharides;
Mice;
Microglia/metabolism*;
RNA-Seq;
Transcriptome
- From:
China Journal of Chinese Materia Medica
2020;45(1):149-156
- CountryChina
- Language:Chinese
-
Abstract:
In this research, high-throughput sequencing was used to investigate the mechanism of Naoxintong Capsules(NXTC) in prevention of post-ischemic inflammation. First, microglia BV-2 inflammatory model was induced by 1.0 μg·mL~(-1) LPS to investigate the effect of intestinal absorption solution of NXTC(NXTCIA) at different concentrations(62.5, 31.25, 15.63, 7.81 μg·mL~(-1)) on LPS-induced BV-2 inflammatory factors in microglia. Then, an RNA-Seq high-throughput sequencing method was performed to identify the differentially expressed mRNAs in microglia BV-2 after pre-treatment with NXTC. GO and KEGG enrichment analysis was used to screen the potential biological processes and related signaling pathways of NXTC in inhibiting inflammation. The results showed that four NXTCIA concentrations could significantly inhibit the release of LPS-induced inflammatory mediators in BV-2 in a dose-dependent manner. Furthermore, high-throughput sequencing results showed that 392 mRNA transcripts were reversed following pre-treatment with NXTC. GO enrichment analysis showed that the transcripts reversed by NXTC were mainly involved in Toll-like receptor signaling pathway, chemokine signaling pathway, and TNF signaling pathway. Taken together, our findings showed that NXTC treatment could provide protective effects against inflammatory response and the mechanism might be related to the regulation of Toll-like receptor signaling pathway, chemokine signaling pathway, and TNF signaling pathway.
