Simultaneous determination of four anthraquinones in Polygonum multiflorum by QAMS.
10.19540/j.cnki.cjcmm.20191014.201
- Author:
Wen-Wen HOU
1
;
Yan-Hong SHI
2
;
Yan SONG
3
;
Yu-Lian ZHANG
3
;
Guang-Hang QU
3
;
Zheng-Tao WANG
4
;
Li YANG
1
Author Information
1. Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine Shanghai 201203, China the MOE Key Laboratory of Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine Shanghai 201203, China.
2. Institute of Traditional Chinese Medicine International Standardization, Shanghai University of Traditional Chinese Medicine Shanghai 201203, China the MOE Key Laboratory of Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine Shanghai 201203, China.
3. SPH Hua Yu Chinese Herbs Co., Ltd. Shanghai 200002, China.
4. the MOE Key Laboratory of Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine Shanghai 201203, China.
- Publication Type:Journal Article
- Keywords:
Polygonum multiflorum;
QAMS;
anthraquinone glycosides;
anthraquinones;
relative correction factors
- MeSH:
Anthraquinones/analysis*;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal/analysis*;
Fallopia multiflora/chemistry*;
Glucosides;
Phytochemicals/analysis*
- From:
China Journal of Chinese Materia Medica
2020;45(1):133-141
- CountryChina
- Language:Chinese
-
Abstract:
A simple, specific and selective quantitative analysis of multi-components by single marker(QAMS) method for simultaneous determination of anthraquinones and anthraquinone glycosides in Polygonum multiflorum was developed. Four main anthraquinones and its glycosides, emodin, emodin-8-O-β-D-glucoside, physcion and physcion-8-O-β-D-glucoside were selected as analytes to evaluate the quality of P. multiflorum. Emodin was used as the internal standard, and the relative correction factors(RCFs) between emodin and the other three anthraquinones were calculated. Comparison of the contents of the four components in 30 batches of P. multiflorum from different regions and 12 batches decoction pieces from different manufacturers by QAMS and external standard method(ESM) showed that there was no significant difference between QAMS and ESM for quantification of the four main components by using relative error results, and the QAMS method was accurate and reliable, and had a good repeatability. In addition, compared with the results calculated by the difference method between total anthraquinone and free anthraquinone in the content determination of P. multiflorum in Chinese Pharmacopoeia, the results of direct determination combined anthraquinone by QAMS were very close to that by measured the external standard method. Therefore, simultaneous quantification of four main anthraquinones by using QAMS is suitable to evaluate the quality of P. multiflorum. Then the optimized assay method of the combined anthraquinone contents showed simple and feasible, which could be replaced and improved the quantification method of the combined anthraquinone in the current Chinese Pharmacopeia.
