Optimization of analysis methods for Astragali Radix and quality evaluation of standard decoction of Astragali Radix.
10.19540/j.cnki.cjcmm.20190630.302
- Author:
Dong-Bo WANG
1
;
Man-Jia ZHAO
2
;
Yun-Tao DAI
2
;
Xue-Mei QIN
3
;
Shi-Lin CHEN
2
Author Information
1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijng 100700, China School of Chemistry and Chemical Engineering, Shanghai University of Engineering Science Shanghai 201620, China.
2. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijng 100700, China.
3. Modern Research Center for Traditional Chinese Medicine, Shanxi University Taiyuan 030006, China.
- Publication Type:Journal Article
- Keywords:
Astragali Radix;
astragaloside Ⅳ;
calycosin;
calycosin 7-O-β-D-glucospyranoside;
solid phase extraction;
standard decoction;
transfer rate
- MeSH:
Astragalus Plant/chemistry*;
Astragalus propinquus;
Drugs, Chinese Herbal/standards*;
Glucosides/analysis*;
Plant Roots/chemistry*;
Quality Control;
Solid Phase Extraction
- From:
China Journal of Chinese Materia Medica
2020;45(1):119-123
- CountryChina
- Language:Chinese
-
Abstract:
Astragali Radix is commonly used as bulk medicinal materials. Chinese Pharmacopoeia contains about 150 compound preparations of Astragali Radix, but the sample preparation method under the determination of Astragali Radix content in Chinese Pharmacopoeia is tedious and time-consuming, not convenient for the test of a large number of samples. Therefore, it is of great significance to simplify the sample preparation method and improve the practicability of the method for the quality control of Astragali Radix and its preparations. In this study, ultrasonic extraction method was used instead of heated reflux extraction, and solid phase extraction method was used to enrich and prepare the samples. A set of practical quality evaluation method was established for Astragali Radix slices and standard decoction, greatly shortening the sample preparation time and improving the accuracy of the method. The results of Astragali Radix standard decoction analysis showed that the transfer rate of calycosin 7-O-β-D-glucospyranoside,(96.5±28.7)%, had great variation, which was found to be related to the conversion of mulberry isoflavone glucoside into calycosin 7-O-β-D-glucospyranoside during the preparation of standard decoction. The transfer rates were(59.4±14.4)% and(101.3±12.3)% for calycosin and astragaloside Ⅳ respectively, which were relatively stable. Therefore, it is suggested that Astragali Radix slices and water decoction preparations should be evaluated by using calycosin and astragaloside Ⅳ as the quality evaluation index. The results provide a scientific and practical method for quality control of Astragali Radix slices and its standard decoction, and also provide scientific evidence for quality evaluation of the preparations.
