Meta-analysis on effect of Grifola frondosa polysaccharide in regulating in vivo immunoregulatory function on animal disease models.
10.19540/j.cnki.cjcmm.20190823.401
- Author:
Ting ZHANG
1
;
Fei ZHAO
2
;
Kai-Nan WU
3
;
Yu JIA
4
;
Xu-Liang LIAO
5
;
Feng-Wen YANG
6
;
Jun-Hua ZHANG
6
;
Bin MA
1
Author Information
1. Evidence Based Medicine Center,School of Basic Medical Sciences,Lanzhou University Lanzhou 730000,China Key Laboratory of Evidence-based Medicine and Clinical Transformation in Gansu Province Lanzhou 730000,China.
2. Medical College,Northwest University for Nationalities Lanzhou 730030,China.
3. the First Clinical Medical College,Lanzhou University Lanzhou 730000,China.
4. School of Stomatology,Lanzhou University Lanzhou 730000,China.
5. Evidence Based Medicine Center,School of Basic Medical Sciences,Lanzhou University Lanzhou 730000,China.
6. Evidence-Based Medicine Center,Tianjin University of Traditional Chinese Medicine Tianjin 300193,China.
- Publication Type:Meta-Analysis
- Keywords:
Grifola frondosa polysaccharide;
Meta-analysis;
animal disease models;
immunoregulatory function
- MeSH:
Animals;
Cytokines/immunology*;
Disease Models, Animal;
Grifola/chemistry*;
Immune System;
Killer Cells, Natural/immunology*;
Macrophages/immunology*;
Mice;
Polysaccharides/pharmacology*;
T-Lymphocytes/immunology*
- From:
China Journal of Chinese Materia Medica
2019;44(23):5174-5183
- CountryChina
- Language:Chinese
-
Abstract:
The study aimed to explore the in vivo immunoregulatory function of Grifola frondosa polysaccharide( GFP) on animal disease models. Databases of PubMed,Embase,Web of Scinece,CNKI,CBM and Wan Fang Data were searched from the date of their establishment to February 2018. Two reviewers independently screened included studies and evaluated their quality by using SYRCLE's risk of bias tool. R software was used to analyze the data. Finally,20 animal experiment studies were included. According to Metaanalysis. For cellular immunity,GFP could effectively enhance the proliferation of effect or T cells,natural killer cells and macrophages in mice. The percentage of CD4+T cells( MD = 1. 89,95% CI [0. 94,2. 83],P < 0. 000 1),CD8+T cells( MD = 8. 46,95% CI[5. 93,11. 00],P<0. 000 1),NK cells( MD= 2. 67,95% CI [0. 23,5. 11],P= 0. 03),and macrophages( MD= 14. 09,95% CI[0. 84,27. 34],P= 0. 04) were all higher than those in control group. For humoral immunity,GFP could increase the secretion of TNF-α and INF-γ． The secretion of TNF-α( SMD = 15. 92,95% CI [9. 07,22. 76],P<0. 000 1) and INF-γ( SMD = 5. 34,95% CI[3. 42,7. 26],P<0. 000 1) were all higher than those in control group. In conclusion,GFP could regulate immunologic function by enhancing the proliferation activity of immune cells( CD4+T cells,CD8+T cells,NK cells and macrophages) and the secretion of immune factors( TNF-α and INF-γ) ． However,it is necessary to further standardize the selection of specific surface markers of immune cells and the administration of GFP,in order to reduce the heterogeneity among the studies. At the same time,more attention shall be paid to experimental design,implementation and full report,especially to the establishment and implementation of animal experimental registration system,so as to improve the transparency and quality of the whole process of animal experimental research,enhance the value of basic research ultimately,and provide a reliable theoretical basis for the transformation of basic research into clinical research.
