Effect of Scutellariae Radix on expression of inflammatory cytokine protein and gene in lung of mice with viral pneumonia caused by influenza virus FM1 infection.
10.19540/j.cnki.cjcmm.201910.09.401
- Author:
Hong-Ri XU
1
;
Ya-Li LI
2
;
Cheng-Xiang WANG
1
;
Guo-Xing LIU
3
;
Chang LIU
3
;
Li-Li ZHANG
3
;
Qing-Mu LI
3
;
Meng LI
4
;
Hong-Yun CAO
3
;
Miao CHENG
5
;
Hong-Ping WANG
2
Author Information
1. Department of Emergency and Intensive Care Unit,the Third Affiliated Hospital of Beijing University of Chinese Medicine Beijing 100029,China Institute of Sepsis,Beijing University of Traditional Chinese Medicine Beijing 100029,China.
2. Department of Emergency,Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine Beijing 100700,China.
3. Department of Emergency and Intensive Care Unit,the Third Affiliated Hospital of Beijing University of Chinese Medicine Beijing 100029,China.
4. Department of Respiratory,Shaanxi Provincial Hospital of Traditional Chinese Medicine Xi'an 710003,China.
5. Department of Respiratory,Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine Beijing 100700,China.
- Publication Type:Journal Article
- Keywords:
RT-PCR;
Scutellariae Radix;
cytokines;
immunohistochemistry;
influenza virus FM1;
pneumonia
- MeSH:
Animals;
Cytokines/immunology*;
Drugs, Chinese Herbal/therapeutic use*;
Lung/virology*;
Mice;
Orthomyxoviridae;
Orthomyxoviridae Infections/drug therapy*;
Pneumonia, Viral/drug therapy*;
Scutellaria baicalensis/chemistry*
- From:
China Journal of Chinese Materia Medica
2019;44(23):5166-5173
- CountryChina
- Language:Chinese
-
Abstract:
Mice models of viral pneumonia were induced by pulmonary adaptive strain FM1 of influenza A virus in Asian mice.RT-PCR and immunohistochemistry were used to dynamically observe the effect of Scutellariae Radix on the protein and gene expression of inflammatory cytokine in the lungs of the model mice infected by influenza virus FM1 at different phases. The partial mechanism of Scutellariae Radix in repairing the immune inflammatory damage of target organs of pneumonia caused by influenza virus was further explored. The results showed that Scutellariae Radix reduced protein and gene expression of proinflammatory cytokines tumor necrosis factor( TNF-α),interleukin IL-1,IL-6 in lung tissues from 3 rd to 5 th day after infection,and increased protein and gene expression of IL-10 and IFN-γ in lung tissues on the 5 th day after infection. Scutellariae Radix may inhibit excessive release of pro-inflammatory cytokines and promote the expression of anti-inflammatory cytokines,thereby inhibiting the systemic inflammatory response syndrome,reducing the immunoinflammatory pathological damage of lung caused by influenza virus FM1 infection,and promoting lung repair of tissue inflammatory lesions.
