Experimental study on effect and mechanism of Danzhi Jiangtang Capsules on diabetic myocardial injury.
10.19540/j.cnki.cjcmm.20191015.401
- Author:
Hui SHI
1
;
Liang WANG
2
;
Zhao-Hui FANG
3
;
Ying-Qun NI
3
;
An-Lu SHEN
4
;
Pei-Pei LIU
4
;
Xiang WANG
1
;
Jin-Ling HUANG
2
Author Information
1. Nursing School,Anhui University of Chinese Medicine Hefei 230012,China.
2. School of Integrated Chinese Medicine and Western Medicine,Anhui University of Chinese Medicine Hefei 230012,China Institute of Integrated Chinese Medicine and Western Medicine,Anhui Academy of Chinese Medicine Hefei 230012,China.
3. the First Clinical Medical College,Anhui University of Chinese Medicine Hefei 230031,China.
4. School of Integrated Chinese Medicine and Western Medicine,Anhui University of Chinese Medicine Hefei 230012,China.
- Publication Type:Journal Article
- Keywords:
Danzhi Jiangtang Capsules;
TLR4/MyD88/NF-κB signaling pathway;
animal model;
diabetic cardiomyopathy;
serum pharmacology
- MeSH:
Animals;
Blood Glucose;
Capsules;
Diabetes Mellitus, Experimental/complications*;
Diabetic Cardiomyopathies/drug therapy*;
Drugs, Chinese Herbal/therapeutic use*;
Myeloid Differentiation Factor 88/metabolism*;
NF-kappa B/metabolism*;
Rats;
Rats, Sprague-Dawley;
Signal Transduction;
Toll-Like Receptor 4/metabolism*
- From:
China Journal of Chinese Materia Medica
2019;44(23):5159-5165
- CountryChina
- Language:Chinese
-
Abstract:
Diabetic cardiomyopathy( DCM) is one of the major cardiovascular complications of diabetes mellitus. Based on the clinical efficacy of Danzhi Jiangtang Capsules( DJC) in the prevention and treatment of diabetes and its cardiovascular complications,both in vivo and in vitro methods were adopted to investigate its effect and underlying mechanism of protecting myocardial injury induced by diabetes. The type 2 diabetic rats were prepared by feeding high-energy food combined with streptozotin( STZ) injection,and the effects of DJC were observed by blood sugar,blood lipid,hemodynamic index,cardiac weight index and the change of cardiac pathological morphology. The protein expressions of TLR4,MyD88 and NF-κB p65 in myocardial tissue were detected and the possible mechanism was preliminarily analyzed. Besides this,DJC containing serum was prepared,H9 c2 cardiomyocyte induced by high sugar were studied to investigate the mechanism of TLR4/MyD88/NF-κB signaling pathway regulating cardiomyocyte injury and the therapeutic effect of DJC. The results demonstrated that fasting blood sugar,glycosylated hemoglobin,total cholesterol and glycerol triglyceride were significantly reduced( P<0. 01,P<0. 05). Cardiac weight index,left ventricle weight index,LVEDP and the protein expressions of TLR4,MyD88 and NF-κB p65 were significantly reduced( P<0. 01,P<0. 05). LVSP,+dp/dtmaxand-dp/dtmaxincreased significantly( P<0. 01,P< 0. 05). Moreover,the pathological damage of myocardial tissue in rats improved significantly. Meanwhile,the protein expressions of TLR4,MyD88 and NF-κB p65 in cardiomyocytes induced by high sugar were significantly inhibited( P<0. 01).It showed that DJC were effective in preventing and treating myocardial injury induced by diabetes and its mechanism may be related to the over-expression of TLR4/MyD88/NF-κB signaling pathway induced by high sugar.
