Effect of processing excipient suet oil on formation and absorption of baohuoside Ⅰ-bile salt self-assembled micelles.
10.19540/j.cnki.cjcmm.20190916.311
- Author:
Hui-Min GU
1
;
E SUN
2
;
Jie LI
3
;
Jian HOU
3
;
Xiao-Bin JIA
4
Author Information
1. Third Clinical Medicine School,Nanjing University of Chinese Medicine Nanjing 210028,China.
2. Third Clinical Medicine School,Nanjing University of Chinese Medicine Nanjing 210028,China Key Laboratory of New Drug Delivery System of Chinese Materia Medica,Jiangsu Provincial Academy of Chinese Medicine Nanjing 210028,China.
3. Key Laboratory of New Drug Delivery System of Chinese Materia Medica,Jiangsu Provincial Academy of Chinese Medicine Nanjing 210028,China.
4. Third Clinical Medicine School,Nanjing University of Chinese Medicine Nanjing 210028,China School of Chinese Materia Medica,China Pharmaceutical University Nanjing 211198,China.
- Publication Type:Journal Article
- Keywords:
Caco-2 monolayer cell model;
baohuoside Ⅰ;
oral bioavailability;
rat intestinal perfusion model;
self-assembled micelles;
suet oil
- MeSH:
Animals;
Caco-2 Cells;
Drugs, Chinese Herbal/pharmacokinetics*;
Epimedium/chemistry*;
Excipients/chemistry*;
Flavonoids/pharmacokinetics*;
Humans;
Intestinal Absorption;
Micelles;
Oils/chemistry*;
Rats
- From:
China Journal of Chinese Materia Medica
2019;44(23):5143-5150
- CountryChina
- Language:Chinese
-
Abstract:
The fried method with suet oil,which can strengthen the effect of Epimedium in warming kidney and enhancing Yang,has been widely used in the processing of Epimedium in traditional Chinese medicine. Based on the formation mechanism of Epimedium flavonoids self-assembled micelles in vivo,the synergistic mechanism of processing excipient suet oil was investigated in this paper from the perspective of pharmaceutics. Baohuoside Ⅰ,as representative component of processed Epimedium,was selected as model drug.Average size and zeta potential were measured and the morphology of micelles was observed under transmission electron microscopy. Caco-2 monolayer cell model,rat intestinal perfusion model and in vivo serum drug concentration method were established to investigate the effect of suet oil on the formation and absorption of the baohuosideⅠ bile salt self-assembled micelles. Baohuoside Ⅰ can form selfassembled micelles under the action of sodium deoxycholate. While,adding suet oil into the baohuoside Ⅰ-bile salt micelles( BSDOC) can make it form a more stable system with a smaller average size,higher Zeta potential,lower polydispersity index( PDI) value,significantly improved encapsulation efficiency and drug loading,indicating that suet oil could significantly improve the micelle formation in vivo. In addition,the permeability coefficient of baohuoside Ⅰ in Caco-2 monolayer cells and the four intestinal organs( duodenum,jejunum,ileum and colon) was increased and the oral bioavailability was also improved after adding the suet oil to BS-DOC.All the results demonstrated that the suet oil can promote the formation and absorption of baohuoside Ⅰ self-assembled micelles,so as to enhance its synergistic effects.
