Survey on pesticide use in Crataegi Fructus and analysis of pesticide residues based on LC-ESI-MS/MS.
10.19540/j.cnki.cjcmm.20190926.201
- Author:
Ting-Ting SUN
1
;
Jiao-Yang LUO
2
;
Jia-An QIN
2
;
Kun-Lun LI
3
;
Jin TIAN
4
;
Shi-Hai YANG
5
;
Mei-Hua YANG
2
Author Information
1. College of Traditional Chinese Medicine,Jilin Agricultural University Changchun 130118,China Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100193,China.
2. Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100193,China.
3. Life Science and Engineering,Southwest Jiaotong University Chengdu 610031,China.
4. Beijing Zhendong Guangming Pharmaceutical Research Institute Beijing 100085,China.
5. College of Traditional Chinese Medicine,Jilin Agricultural University Changchun 130118,China.
- Publication Type:Journal Article
- Keywords:
Crataegi Fructus;
LC-ESI-MS/MS;
pesticide residue;
survey
- MeSH:
Chromatography, Liquid;
Crataegus/chemistry*;
Drug Contamination;
Drugs, Chinese Herbal/analysis*;
Pesticide Residues/analysis*;
Surveys and Questionnaires;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2019;44(23):5088-5093
- CountryChina
- Language:Chinese
-
Abstract:
In order to study the pesticide residues of the medicinal Crataegi Fructus,this study aims to establish an analysis method for pesticide residues( mainly containing insecticides and fungicides) suitable for the actual situation of medicinal Crataegi Fructus based on the survey of the pesticides of the Crataegi Fructus base,combined with the blind screening results of the LC-ESI-MS/MS pesticide screening platform established by the research team in the early stage. Then,the pesticide residues in medicinal Crataegi Fructus from Shandong,Hebei,Henan,Shanxi,and Liaoning( main cultivation areas) were analyzed. The samples were pretreated by the modified Qu ECh ERS method,i.e.,extracted with acetonitrile-water( 9 ∶1),purified by PSA,C_(18),GCB,silica gel. The detection of pesticides was performed by LC-MS/MS. The ion source was ESI with positive scanning mode,and the linearity of 11 kinds of pesticides in the range of 5-300 μg·kg~(-1) was acceptable( R~2>0. 996 9). All the recoveries of pesticides were within 70. 02%~(-1)12. 0% in the low,medium and high levels,with RSD≤17%. The results showed that the detection rate of carbendazim,chlorpyrifos and difenoconazole is 79%,82%,56%,respectively. Besides,the prohibition pesticide carbofuran were detected in some of the batches,indicating the security risk. This study provides methodological references and basic data for risk assessment of Crataegi Fructus and government regulation.
