High-throughput screening of ochratoxin A in Chinese herbal medicines using enzyme-linked immunoassay.
10.19540/j.cnki.cjcmm.20190916.201
- Author:
Lu QIN
1
;
Lei ZHANG
2
;
Jia-Yi JIANG
2
;
Chang-Jian WANG
2
;
Xiao-Wen DOU
2
;
Li WAN
3
;
Mei-Hua YANG
2
Author Information
1. College of Pharmacy,Chengdu University of Traditional Chinese Medicine Chengdu 611137,China Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine,Ministry of Education,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100193,China.
2. Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine,Ministry of Education,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100193,China.
3. College of Pharmacy,Chengdu University of Traditional Chinese Medicine Chengdu 611137,China.
- Publication Type:Journal Article
- Keywords:
Curcumae Longae Rhizoma;
Myristicae Semen;
Zingiberis Rhizoma;
indirect competitive enzyme-linked immunosorbent assay;
ochratoxin A;
rapid screening
- MeSH:
Chromatography, Liquid;
Drug Contamination;
Drugs, Chinese Herbal/analysis*;
Enzyme-Linked Immunosorbent Assay;
High-Throughput Screening Assays;
Ochratoxins/analysis*;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2019;44(23):5072-5077
- CountryChina
- Language:Chinese
-
Abstract:
An indirect competitive enzyme-linked immunosorbent assay( ic-ELISA) was developed for the rapid detection of ochratoxin A( OTA) in nutmeg( Myristicae Semen),ginger( Zingiberis Rhizoma) and turmeric( Curcumae Longae Rhizoma). The matrix matching standard curve was used instead of the standard curve of sample diluent,and the sample extract and sample diluent were optimized. The sensitivity( IC_(50)) of this method for OTA in nutmeg,ginger and turmeric were determined as 0. 146,0. 157 and 0. 153 ng·m L~(-1),respectively and the limits of detection( LODs) were 0. 040,0. 032 and 0. 031 ng·m L~(-1),respectively. The recovery of samples ranged from 75. 99% to 122. 3%,with RSD<10%. Two positive samples for nutmeg and one positive sample for turmeric occurred in 50 samples,and the highest OTA contamination value was 1 167. 8 μg·kg~(-1). The results were further confirmed by LC-MS/MS. It shows that the developed ic-ELISA method is simple,rapid and sensitive,and can be applied for rapid and high-throughput screening of OTA in nutmeg,ginger and turmeric,as well as some other CHMs.
