Quality evaluation of multi-components simultaneous determination and fingerprint of Gardeniae Fructus from different regions.
10.19540/j.cnki.cjcmm.20190521.201
- Author:
Hong-Hong CAO
1
;
Wei-Hua YAN
1
;
Shuang GUO
1
;
Min HAO
1
;
Ke-Wei ZHANG
1
;
Tu-Lin LU
1
;
Chun-Qin MAO
1
;
Lin LI
1
;
Yan SONG
2
Author Information
1. Nanjing University of Chinese Medicine Nanjing 210023,China.
2. Shanghai Huayu Pharmaceutical Co.,Ltd. Shanghai 200002,China.
- Publication Type:Journal Article
- Keywords:
Gardeniae Fructus;
determination of multi-component content;
fingerprint;
multivariate statistical analysis;
region
- MeSH:
China;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal/standards*;
Fruit/chemistry*;
Gardenia/chemistry*;
Phytochemicals/analysis*
- From:
China Journal of Chinese Materia Medica
2019;44(18):4026-4033
- CountryChina
- Language:Chinese
-
Abstract:
An analysis method was established by UPLC fingerprint and then applied to simultaneous determination of multiple compounds in Gardeniae Fructus from different areas in China. Samples were separated on a Waters Acquity UPLC BEH C18( 2. 1 mm×50 mm,1. 7 μm) column with 0. 1% formic acid-water and acetonitrile solution as gradient mobile phase at a flow rate of 0. 4 m L·min-1 at various wavelengths. The similarity of samples was over 0. 95 with ″Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine( 2012 edition) ″. The UPLC common fingerprints for 32 batches were established with 19 common peaks identified. The samples were divided into 3 groups analyzed by HCA and PCA. Five components were identified as the main compositions which caused the differences of chemical constituents in the samples from different areas with partial least squares discriminant analysis( PLS-DA). The content of the total components in each area was Zhejiang > Fujian > Jiangxi > Sichuan. This method was accurate and viable,could be used to evaluate the quality of Gardeniae Fructus.
