Isolation and identification of pathogen of Dendrobium officinale gray mold and its prevention and control.
10.19540/j.cnki.cjcmm.2019730.101
- Author:
Jing-Mao YOU
1
;
Jie GUO
1
;
Zhe LI
2
;
Qin YANG
3
;
Yuan-Yuan DUAN
1
;
Xiao-Liang GUO
1
;
Da-Ye HUANG
4
;
Zhuang-Ling ZOU
1
;
Han-Jiu GUO
1
Author Information
1. Institute of Chinese Herbal Medicines,Hubei Academy of Agricultural Sciences Enshi 445000,China.
2. Biocentury Transgene ( China) Co.,Ltd. Shenzhen 518048,China.
3. University Hospital of Hubei University for Nationalities Enshi 445000,China.
4. Hubei Biopesticide Engineering Research Center Wuhan 430064,China.
- Publication Type:Journal Article
- Keywords:
Botrytis cinerea;
Dendrobium officinale;
control;
identification
- MeSH:
Alkaloids;
Botrytis/pathogenicity*;
Coumarins;
Dendrobium/microbiology*;
Eugenol;
Fungicides, Industrial;
Plant Diseases/prevention & control*;
Plant Leaves/microbiology*;
Quinolizines;
Matrines
- From:
China Journal of Chinese Materia Medica
2019;44(18):3954-3959
- CountryChina
- Language:Chinese
-
Abstract:
Through investigation,it was found that the main disease of leaves was grey mold on Dendrobium officinale in Hubei province,which has a great impact on the yield and quality of D. officinale. The identification of morphological and molecular biological was used to prove that the pathogen was Botrytis cinerea. Through test the effect of 5 plant source fungicides and 4 antibiotic fungicides on mycelial growth of strain HS1,which proved 0. 3% eugenol had the best inhibitory effect,EC50 was 0. 29 mg·L-1,the second was1% osthol and EC50 was 1. 12 mg·L-1,the EC50 of 0. 5% matrine was 9. 16 mg·L-1,the EC50 of the other six fungicides was higher than 10 mg·L-1. The field control effect test proved that 0. 3% eugenol had the best control effect,reaching 89. 44%,secondly for 1%osthole,which was 77. 17%,0. 5% matrine was in the third place with 62. 37% of effective rate. However,the control effect of the other fungicides was less than 60%. The three plant-derived fungicides were safe for the produce of D. officinale and showed no phytotoxicity. The effect of these fungicides on the growth of D. candidum was tested,and proved that all the fungicides were safe and harmless to D. candidum. This study provides a research basis for the safe and effective prevention and control gray mold of D. officinale.
