Screening and identification of antagonistic Bacillus against Astragalus membranaceus root rot and its effect on microorganism community in root zone soil.
10.19540/j.cnki.cjcmm.20190701.108
- Author:
Fen GAO
1
;
Xiao-Xia ZHAO
1
;
Huan YAN
2
;
Zhen-Hong LEI
3
;
Meng-Liang WANG
1
;
Xue-Mei QIN
2
Author Information
1. Institute of Applied Chemistry,Shanxi University Taiyuan 030006,China.
2. Modern Research Center for Traditional Chinese Medicine,Shanxi University Taiyuan 030006,China.
3. Shanxi Zhendong Geo-herbals Development Co.,Ltd. Changzhi 047100,China.
- Publication Type:Journal Article
- Keywords:
Astragalus membranaceus var.mongholicus;
antagonistic microorganism;
bacterial diversity;
multiplescreening;
regulation;
root rot
- MeSH:
Astragalus Plant/microbiology*;
Bacillus/physiology*;
Biological Control Agents;
Fusarium/pathogenicity*;
Plant Diseases/prevention & control*;
Plant Roots/microbiology*;
Soil Microbiology
- From:
China Journal of Chinese Materia Medica
2019;44(18):3942-3947
- CountryChina
- Language:Chinese
-
Abstract:
The Astragalus membranaceus root rot disease,a soil-borne disease,has become increasingly severe in Shanxi province.This study was aimed at getting antagonistic Bacillus with excellent bio-control effects,and determining its effects on bacterial communities in root zone soil. With Fusarium solani and F. acuminatum as the target,antagonistic Bacillus was selected through such tests as living body dual culture,antifungal effect of bacteria-free filtrate,mycelia growth inhibition in vitro and control effect in detached roots,and identified with morphology,physio-biochemical characteristics and 16 S r DNA sequence analysis. The results showed that the Bacillus strain SXKF16-1 had obvious antifungal effect. The diameter of inhibition zone of its bacteria-free filtrate to F. solani and F. acuminatum was( 25. 90±1. 18) mm and( 25. 86±1. 85) mm respectively,and showed a lasting inhibition effect to mycelia growth. The disease index of the protective treatment and that of the cure treatment in detached roots test to F. solani and F. acuminatum were( 37. 50±8. 58),( 41. 67±4. 90) and( 25. 00±8. 33),( 38. 89±9. 62) respectively,both being significantly different( P<0. 05) from that of the control. The strain SXKF16-1 was identified as Bacillus atrophaeus. The B. atrophaeus SXKF16-1 showed significantly inhibition effect to pathogen causing root rot and could increase the bacterial diversity in root zone soil. It has potential to be developed as a special biocontrol agent.
