Advances in synthesis of artemisinin based on plant genetic engineering.
10.19540/j.cnki.cjcmm.20190416.405
- Author:
Chao ZHANG
1
;
Yao ZHU
1
;
Xiao-Pu YIN
1
;
Qiu-Hui WEI
1
;
Na-Na ZHANG
1
;
Chen-Xi LI
1
;
Tian XIE
1
;
Rong CHEN
1
Author Information
1. School of Medicine,Hangzhou Normal University Hangzhou 310036,China Key Laboratory of Elemene Class Anti-cancer Chinese Medicine of Zhejiang Province Hangzhou 310036,China Engineering Laboratory of Development and Application of Traditional Chinese Medicine from Zhejiang Province Hangzhou 310036,China.
- Publication Type:Journal Article
- Keywords:
Artemisia annua;
artemisinin;
biosynthetic pathway;
plant genetic engineering
- MeSH:
Antimalarials;
Artemisia annua;
Artemisinins;
Metabolic Engineering;
Transcription Factors
- From:
China Journal of Chinese Materia Medica
2019;44(19):4285-4292
- CountryChina
- Language:Chinese
-
Abstract:
Artemisinin is a kind of sesquiterpene lactone containing endoperoxide bridge,which is the most effective anti-malarial drug at present. However,low content of artemisinin in Artemisia annua,ranging from 0. 1%-1. 0% of dry weight,as well as the complicated extraction process have resulted in low yield and high cost of artemisinin,making it difficult to meet market demand.Based on the development of high-throughput sequencing and molecular biology,the related enzyme genes and transcription factors involved in the artemisinin metabolic pathway were cloned and identified. Metabolic engineering and synthetic biology methods to modify the original metabolic pathway of A. annua and genetic engineering in heterologous host cells have become one of the hotspots in this field. Therefore,the molecular mechanism of artemisin biosynthesis,different strategies of genetic modifications of A. annua,and the research status and application prospect of artemisinin synthesis in heterologous host cells( Nicotiana benthamiana,Physcomitrella patens) were summarized in our review,hoping to provide molecular basis and theoretical basis for breeding new varieties of A. annua with high artemisinin output.
