Preparation of angiopep-2-modified FITC-labeled neurotoxin nanoparticles and in vitro release characteristics.
10.19540/j.cnki.cjcmm.20190630.301
- Author:
Wu-Chao LI
1
;
Li-Li YAN
2
;
Xiao-Jie CHEN
3
;
Fan-Zhu LI
3
;
Zhi-Hong ZHU
3
Author Information
1. Inspection Division Ⅲ ,Center for Food and Drug Inspection of NMPA Beijing 100044,China School of Pharmacy,Zhejiang Chinese Medical University Hangzhou 311400,China.
2. Drug Registration and Evaluation Department,Center for Certification and Evaluation,Guangdong Drug Administration Guangzhou 510000,China.
3. School of Pharmacy,Zhejiang Chinese Medical University Hangzhou 311400,China.
- Publication Type:Journal Article
- Keywords:
FITC;
Mal-PEG-PLA block copolymer;
angiopep-2;
in vitro release;
nanoparticles;
neurotoxin
- MeSH:
Drug Carriers;
Fluorescein-5-isothiocyanate;
Nanoparticles;
Particle Size;
Peptides;
Polyethylene Glycols
- From:
China Journal of Chinese Materia Medica
2019;44(19):4171-4178
- CountryChina
- Language:Chinese
-
Abstract:
In order to prepare angiopep-2 modified fluorescein isothiocyanate-labeled neurotoxin nanoparticles( ANG-NPs/FITCNT),emulsion/solvent evaporation method was used with m PEG-PLA and ANG-PEG-PLA( in proper proportions) as carriers and with FITC-NT as drug. With particle size and encapsulation efficiency as comprehensive indexes,the effects of different ultrasound power and ultrasound time combinations on the process were investigated. The in vitro release characteristics of nanoparticles in PBS buffer at p H 7. 4 and p H 6. 5 were investigated by dialysis method. The results indicated that the optimum process for preparing ANG-NPs/FITC-NT was as follows: ultrasonic power 90 W,ultrasonic time 30 s. In such optimal process,ANG-NPs/FITC-NT were well-shaped under the transmission electron microscope,with an average particle size of( 123. 9±0. 5) nm,Zeta potential of(-10. 5±0. 5) m V,encapsulation efficiency of( 68. 1±0. 4) %,and the drug loading of( 0. 82±0. 01) %. The in vitro drug release profiles of the nanoparticles in PBS buffer at p H 7. 4 and p H 6. 5 were both consistent with Ritger-Peppas equation,ln Q = 0. 508 8 lnt-2. 285 0,r = 0. 961 5( p H 7. 4) and ln Q= 0. 449 9 lnt-1. 855 3,r = 0. 970 3( p H 6. 5),respectively. The experiment results proved that the nanoparticles prepared by emulsion/solvent evaporation method had uniform particle size,high encapsulation efficiency and in vitro sustained release characteristic,which might be a potential carrier for NT intracerebral drug delivery.
