Isolation and Identification of the Antigen Recognized by Human Cytomegalovirus Specific Monoclonal Antibody SCMVM 34.
- Author:
Chung Gyu PARK
;
Yoon Hoh KOOK
;
Chang Yong CHA
;
Eung Soo HWANG
;
Dong Gyun LIM
;
Ju Young SEOH
;
Jae Won PARK
;
Hyun Soon JONG
- Publication Type:Original Article
- MeSH:
Chromatography;
Chromatography, High Pressure Liquid;
Chromatography, Ion Exchange;
Cytomegalovirus*;
Electrophoresis, Polyacrylamide Gel;
Humans*;
Molecular Weight;
Peptide Fragments;
Peptides;
Sequence Analysis, Protein
- From:Journal of the Korean Society for Microbiology
1997;32(3):325-334
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Human cytomegalovirus (HCMV)-specific monoclonal antibody, SCMVM 34, recognizes early antigen confined to the nucleus of HCMV-infected cells. This study was performed to identify the antigen reactive to SCMVM 34 with purification and amino acid sequencing. The nuclear and cytoskeletal fraction of HCMV-infected cells was subjected to 0.4 M NaCl extraction, DEAE-Sephacel ion exchange chromatography, DNA-cellulose chromatography and SDS-PAGE. The molecular weight of the reactive proteins was 52 kD, 40 kD and 34 kD. The modified or blocked amino termini of 52 kD and 40 kD showed resistance to Edman degradation. The internal peptide fragments were isolated by tryptic digeytion and reverse-phase HPLC. The internal amino acid sequence analysis of the peptides from HPLC profile revealed that the antigens recognized by SCMVM 34 was ppUIA4.
