Research on hemostatic effect and mechanism of carbonized Scutellariae Radix on rats of uterine bleeding.
10.19540/j.cnki.cjcmm.20190826.401
- Author:
Qi HUANG
1
;
Li-Li LI
2
;
Chun-Song CHENG
3
;
Cun ZHANG
4
;
Chuan-Shan JIN
1
Author Information
1. College of Pharmacy,Anhui University of Chinese Medicine Hefei 230012,China Anhui Province Key Laboratory of Traditional Chinese Medicine Decoction Pieces of New Manufacturing Technology Heifei 230012,China.
2. College of Pharmacy,Anhui University of Chinese Medicine Hefei 230012,China.
3. Macau University of Science and Technology Macau 999078,China.
4. Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China.
- Publication Type:Journal Article
- Keywords:
carbonized Scutellariae Radix;
hemostatic effect;
inflammatory reaction;
renovation of endometrium;
uterine bleeding
- MeSH:
Animals;
Drugs, Chinese Herbal/pharmacology*;
Female;
Hemostatics/pharmacology*;
Humans;
Inflammation;
Interleukin-1beta;
Rats;
Scutellaria baicalensis;
Tumor Necrosis Factor-alpha;
Uterine Hemorrhage
- From:
China Journal of Chinese Materia Medica
2019;44(21):4691-4697
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this paper was to investigate the hemostatic effect and mechanism of carbonized Scutellariae Radix on uterine bleeding in the rats caused by early pregnancy termination. Eight unpregnant female rats were selected as normal group. Forty female rats conceived on the same day received mifepristone(11. 4 mg·kg-1) and misoprostol(125 μg·kg-1) to induce model of incomplete abortion in early pregnancy. Abortion models were randomly divided into model group,carbonized Scutellariae Radix water extract low dose group(0. 55 g·kg-1),medium group(1. 10 g·kg-1),high dose group(2. 20 g·kg-1) and positive control group(0. 45 g·kg-1).The uterine bleeding volume was detected by ultraviolet spectrophotometry. The pathological changes of endometrium were detected by HE(hematixylin-eosin) staining. The levels of interleukin(IL-1β),IL-6 and tumor necrosis factor(TNF-α) in the plasma of rats were determined by ELISA. The expression levels of IL-1β,IL-6 and TNF-α mRNA in the uterus of rats were determined by RT-PCR.The protein expression levels of VEGF,MMP-2 and MMP-9 were determined by Western blot. As compared with the normal group,the uterine bleeding volume and histopathological score were increased significantly; microvessel density of endometrial tissues was decreased significantly; the contents of TNF-α,IL-1β and the levels of TNF-α mRNA and IL-1β mRNA in the plasma were increased,while the content of IL-6 and level of IL-6 mRNA were decreased significantly. The protein expression levels of VEGF,MMP-2 and MMP-9 in the uterine tissues were also decreased. As compared with the model group,the uterine bleeding volume was decreased significantly in the carbonized Scutellariae Radix medium dose and high dose groups; endometrial repair was promoted,and the microvessel density of endometrial tissues was increased significantly; the contents of IL-1β and TNF-α in the plasma of rats were decreased significantly,while the content of IL-6 in the plasma of rats was increased significantly; the expression levels of IL-1β and TNF-α mRNA in the uterus of rats were decreased and the expression level of IL-6 mRNA showed an increase; the protein expressions of VEGF,MMP-2 and MMP-9 were decreased significantly in carbonized Scutellariae Radix medium and high dose groups. In conclusion,carbonized Scutellariae Radix showed good hemostatic effect,and its mechanism may be related to the repair of endometrium and inhibition of inflammatory reaction.
