Study on HPLC fingerprint of Sabia parviflora.
10.19540/j.cnki.cjcmm.20190625.201
- Author:
Wen-Fen XU
1
;
Guo-Ji PAN
1
;
Qing-Wen SUN
1
;
Cai-Hong BAI
1
;
Meng-Ge LIU
1
;
Qu LIU
1
;
Tian-Jin YANG
1
Author Information
1. Guizhou University of Traditional Chinese Medicine Guiyang 550025,China.
- Publication Type:Journal Article
- Keywords:
HPLC;
Sabia parviflora;
cluster analysis;
fingerprint;
principal component analysis
- MeSH:
Chromatography, High Pressure Liquid;
Cluster Analysis;
Drugs, Chinese Herbal;
Principal Component Analysis;
Quality Control
- From:
China Journal of Chinese Materia Medica
2019;44(21):4670-4676
- CountryChina
- Language:Chinese
-
Abstract:
This research was performed to establish the HPLC fingerprint of Sabia parviflora. HPLC method was carried out on a Thermo Accucore-C18(4. 6 mm×150 mm,2. 6 μm) column by 30% tetrahydrofuran in methyl alcohol-acetonitrile-0. 1% phosphate solution as mobile phase at a flow rate of 1. 0 m L·min-1,the column temperature was 30 ℃ and the detection wavelength was 360 nm. The fingerprints were further evaluated by chemometrics methods including similarity analysis,hierarchical clustering analysis,and principal component analysis. In HPLC fingerprint,15 common peaks were selected as the common peaks,and 6 contents of them were identified. The similarity degrees of 38 batches of the samples was more than 0. 710,and the samples were divided into 6 clusters by their quality difference. The method was precision,repeatable,stable,simple and reliable,which could be used for quality control and evaluation of S. parviflora.
