Analysis of constituents from different parts of Callicarpa nudiflora by UPLC-Q-TOF-MS/MS.
10.19540/j.cnki.cjcmm.20190906.202
- Author:
Bo YI
1
;
Min ZHANG
2
;
Yin-Ming HU
2
;
Tao CHEN
2
;
Mao-Yuan WANG
3
;
Shi-Xiu FEGN
2
Author Information
1. 928 Hospital of PLA Haikou 571159,China.
2. Key Laboratory of Southern Subtropical Plant Diversity,Shenzhen Fairy Lake Botanical Garden,Chinese Academy of Sciences Shenzhen 518004,China.
3. Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences Haikou 571101,China.
- Publication Type:Journal Article
- Keywords:
Callicarpa nudiflora;
OPLS-DA;
PCA;
chemical component;
different parts;
green leaves
- MeSH:
Callicarpa;
Chromatography, High Pressure Liquid;
Chromatography, Liquid;
Flavonoids;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2019;44(21):4661-4669
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to clarify the main components of the green leaves of Callicarpa nudiflora,and to compare the difference of main components between the green leaves,yellow leaves,branches and seeds. In this study,ultra-high performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS/MS) coupled with the UNIFI scientific information system was adopted. And the identification of the main chemical components of C. nudiflora was combined with reference materials,literatures and online database. In addition,the difference of main components was analyzed by Progenesis QI,principal component analysis(PCA) and orthogonal partial least squares discriminant(OPLS-DA). A total of 57 compounds were identified in green leaves,including phenylpropanoids,flavonoids and iridoids. Among them,the relative content of phenylethanoid glycosides was highest. Furthermore,the PCA analysis showed that there are significant differences in main components of the branches and other parts of C. nudiflora. Combined with OPLS-DA analysis,nudifloside,parvifloroside B and β-hydroxysamioside were selected as the characteristic components for distinguish the leaves and branches of C. nudiflora. Our study provided a scientific basis for the collection and identification of C. nudiflora.
