Evaluation of key production processes of Chan Taoren formula granules based on characteristic chromatogram and D-amygdalin transfer rate.
10.19540/j.cnki.cjcmm.20190804.307
- Author:
Ke-Qing ZHANG
1
;
Yan CHEN
1
;
Jia-Nan WANG
1
;
Hui-Jie JIANG
1
;
Xin XU
1
;
Xin-Gang DU
2
;
Hui ZHANG
1
;
Ji-Zhong YAN
1
Author Information
1. College of Pharmaceutical Sciences,Zhejiang University of Technology Hangzhou 310014,China.
2. Anhui Jiuzhou Fangyuan Pharmaceutical Co.,Ltd. Bozhou 236800,China.
- Publication Type:Journal Article
- Keywords:
Chan Taoren;
D-amygdalin;
characteristic chromatogram;
formula granules;
transfer rate
- MeSH:
Amygdalin;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
Quality Control;
Water
- From:
China Journal of Chinese Materia Medica
2019;44(21):4641-4647
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed to establish characteristic chromatogram and content determination method for Chan Taoren formula granules,evaluate the production processes of Chan Taoren formula granules based on the correlation of characteristic chromatogram and the transfer rate of D-amygdalin,and clarify the key control points. The optimized analytical method was carried out on a Waters CORTECS C18 column(4. 6 mm×150 mm,2. 7 μm) with acetonitrile-0. 1% phosphoric acid aqueous solution as the mobile phase at a flow rate of 0. 6 m L·min-1. The detection wavelength was 207 nm,and the column temperature was 20 ℃ ． As compared with the standard decoction of Chan Taoren,there were five characteristic peaks in the decoction pieces,extracts,concentrates,spray-dried powders and formula granules,basically consistent in relative retention time and peak pattern; in addition,the transfer rate of D-amygdalin from Chan Taoren pieces to the formula granules was within the transfer rate range of standard decoction. The average transfer rate of D-amygdalin was 56.65%,72.85%,94.58% and 99.29% respectively in the extraction,concentration,spray drying and granulation processes. Therefore,the factors affecting D-amygdalin in the extraction process were further studied. The results showed that D-amygdalin was easily converted to L-amygdalin in the water extraction process,leading to a low transfer rate of D-amygdalin in this process.D-amygdalin was unstable under alkaline conditions and prone to isomerization. Both liquid to solid ratio and extraction time had significant effects on the extraction rate of D-amygdalin. In this study,the key links in the production process of Chan Taoren formula granules was clarified based on the characteristic chromatogram and the quantity transmission of D-amygdalin,which provided a theoretical basis for production and quality control.
