Brain lipidomics of intervention effect of Salviae Miltiorrhizae Radix et Rhizoma and Chuanxiong Rhizoma on ischemic stroke based on UPLC-Q-TOF-MS technique.
10.19540/j.cnki.cjcmm.20190321.404
- Author:
Ying-Feng ZHANG
1
;
Yuan-Shi WEI
1
;
Xing-Xing HUANG
1
;
Li-Xia ZHU
2
Author Information
1. College of Chinese Materia Medica,Guangzhou University of Chinese Medicine Guangzhou 510006,China.
2. Department of Traditional Chinese Medicine,Zhujiang Hospital,South Medical University Guangzhou 510282,China.
- Publication Type:Journal Article
- Keywords:
Chuanxiong Rhizoma;
Salviae Miltiorrhizae Radix et Rhizoma;
UPLC-Q-TOF-MS;
ischemic stroke;
lipidomics
- MeSH:
Animals;
Brain;
Brain Ischemia;
Drugs, Chinese Herbal;
Lipidomics;
Rats;
Stroke
- From:
China Journal of Chinese Materia Medica
2019;44(20):4511-4518
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this paper was to investigate the intervention effect of Salviae Miltiorrhizae Radix et Rhizoma and Chuanxiong Rhizoma on brain lipid metabolism in rats with ischemic stroke. The ischemic stroke rat model was established by middle cerebral artery occlusion( MCAO) method. The brain tissues were collected after the last administration with Salviae Miltiorrhizae Radix et Rhizoma and Chuanxiong Rhizoma decoction lyophilizate. UPLC-Q-TOF-MS was used to carry out the brain lipidomics study. The lipidomics data were processed with the OPLS-DA model to find out the lipid regulation effect of Salviae Miltiorrhizae Radix et Rhizoma combined with Chuanxiong Rhizoma on ischemic stroke. The results showed that Salviae Miltiorrhizae Radix et Rhizoma and Chuanxiong Rhizoma decoction lyophilized powder can significantly alleviate brain lipidomics profiles in middle cerebral artery occlusion model rats. Eleven differential lipid metabolites in ischemic stroke model were identified. In this experiment,the protective effects of Salviae Miltiorrhizae Radix et Rhizoma and Chuanxiong Rhizoma decoction lyophilized powder on cerebral ischemia injury was verified,which might be related to the regulation of abnormal lipid metabolism.
