Identification of compounds in Lycii Cortex by UPLC-LTQ-Orbitrap-MS.
10.19540/j.cnki.cjcmm.20190506.202
- Author:
Xiao-He CHEN
1
;
Lei SU
1
;
Li-Juan JIANG
1
;
Wei ZHANG
1
;
Yan-Yan JIANG
1
;
Bin LIU
1
Author Information
1. School of Chinese Pharmacy,Beijing University of Chinese Medicine Beijing 102488,China.
- Publication Type:Journal Article
- Keywords:
Lycii Cortex;
UPLC-LTQ-Orbitrap;
chemical constituents;
fragmentation rules;
phenolic amides
- MeSH:
Chromatography, High Pressure Liquid;
Coumarins;
Drugs, Chinese Herbal/chemistry*;
Mass Spectrometry;
Phenols
- From:
China Journal of Chinese Materia Medica
2019;44(20):4486-4494
- CountryChina
- Language:Chinese
-
Abstract:
This experiment was performed to analyze and identify the chemical constituents of Lycii Cortex by UPLC-LTQ-OrbitrapMS. The analysis was performed on a Waters Xbridge Shield RP18( 4. 6 mm×250 mm,5 μm) column with the mobile phase of 0. 1%formic acid( A)-acetonitrile( B) under gradient conditions at a flow rate of 1. 0 m L·min-1 and the temperature maintained at 35 ℃ ．Electrospray ionization ion trap time-off light multistage mass spectrometry was applied for qualitative analysis under positive and negative ion modes. The results indicated that 55 compounds consisted of 39 phenolic amides,6 organic acids,3 cyclic peptides,2 coumarins and 5 others. In conclusion,an UPLC-LTQ-Orbitrap-MS method was established to qualitative analysis of Lycii Cortex in this study,and the fragmentation rules of phenolic amides were summarized,which provides a good foundation for further study of Lycii Cortex.
