Studies on active aromatic constituents from rhizomes of Sophora tonkinensis.
10.19540/j.cnki.cjcmm.20190520.202
- Author:
Hao-Ke HE
1
;
Hua SUN
1
;
Sheng-Li YANG
1
;
Meng-Yin TIAN
1
;
Dan ZHANG
1
;
Su-Juan WANG
1
Author Information
1. State Key Laboratory of Bioactive Substance and Function of Natural Medicines,Institute of Materia Medica,Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100050,China.
- Publication Type:Journal Article
- Keywords:
Sophora tonkinensis;
aromatic constituents;
hepatoprotection
- MeSH:
Chromatography, High Pressure Liquid;
Hep G2 Cells;
Humans;
Rhizome/chemistry*;
Sophora/chemistry*
- From:
China Journal of Chinese Materia Medica
2019;44(20):4481-4485
- CountryChina
- Language:Chinese
-
Abstract:
Aromatic constituents from rhizomes of Sophora tonkinensis were purified by extensive chromatographic techniques including column chromatography over macroporous resin,MCI,silica gel,weak acid cation exchange resin,Sephadex LH-20,ODS,and semi-preparative HPLC. Twelve aromatic compounds were isolated and identified from the water aqueous extract of the rhizomes of S.tonkinensis. Their structures were elucidated as 4-( 3-hydroxypropyl) phenol( 1),( ±)-4-( 2-hydroxypropyl) phenol( 2),benzamide( 3),( ±)-3-( p-methoxyphenyl)-1,2-propanediol( 4),4-methoxybenzamide( 5),3-hydroxy-1-( 4-hydroxy-3-methoxyphenyl) propan-1-one( 6),tyrosol( 7),( ±)-2,3-dihydroxypropyl benzoate( 8),vanillin alcohol( 9),7,3'-dihydroxy-8,4'-dimethoxyisoflavone( 10),7,4'-dihydroxy-3'-methoxyisoflavone( 11),and 7,3'-dihydroxy-5'-methoxyisoflavone( 12). Compounds 1-9 were firstly isolated from the Sophora genus. Compounds 4,5,10 and 11 can remarkably protect Hep G2 cell against APAP-induced damage at the concentration of 10 μmol·L-1. Compounds 1-12 exhibited no significant activities on the assays of inhibition of LPS-induced NO production in RAW cell lines and NF-κB inhibition.
