Identification and evaluation of Citrus grandis based on DNA barcode,UPLC and chromaticity method.
10.19540/j.cnki.cjcmm.20190729.103
- Author:
Hai-Sheng LU
1
;
Ting LI
2
;
Dan JIANG
2
;
Yu-Feng SUN
2
;
Xiao-Xi CHANG
2
;
Xiao-Song HU
2
;
Chun-Sheng LIU
2
Author Information
1. Wangjing Hospital,China Academy of Chinese Medical Sciences Beijing 100102,China.
2. School of Traditional Chinese Medicine,Beijing University of Chinese Medicine Beijing 100029,China.
- Publication Type:Journal Article
- Keywords:
Citrus grandis;
ITS2;
UPLC;
chromaticity
- MeSH:
Apigenin;
Citrus/genetics*;
DNA Barcoding, Taxonomic;
Drugs, Chinese Herbal
- From:
China Journal of Chinese Materia Medica
2019;44(20):4419-4425
- CountryChina
- Language:Chinese
-
Abstract:
In order to identify the source of Citrus grandis and evaluate its quality originate from two areas comprehensively,DNA barcode was used to identify 26 samples of C. grandis. The content of naringin,rhoifolin,naringenin and apigenin was determined by UPLC method,and the color difference was numerically studied by color difference analyzer,which was related to the effective components of C. grandis. The results showed that samples was the source of C. grandis in both regions. The ITS2 sequence length was about400-500 bp,and the sequence similarity reached 99. 82%. There was only one base deletion in the two groups. There was one base A in some medicinal materials of Guangdong at 330 bp,but no base in Chongqing. The contents of naringin and rhoifolin in Chongqing samples were higher than those in Guangdong samples,and there were statistical differences between naringenin and apigenin. The chroma value showed that L*value of Guangdong was larger,a*value was smaller,L*value of Chongqing was smaller,and a*value was larger,while the b*value of both was not significantly different; The results of correlation analysis showed that naringin,rhoifolin,naringenin were positively correlated with L*,b*value,negatively correlated with a*value,and apigenin had no correlation with L*,a*,b*value. In this study,the scientific identification and evaluation of C. grandis was carried out to provide a new idea for the further study of the rapid identification and evaluation of C. grandis.
