Effects of processing on pharmacokinetics of Descurainiae Semen's constituents in rats.
10.19540/j.cnki.cjcmm.20190823.203
- Author:
Xian-Kuo YU
1
;
Han-Yan LUO
1
;
Ya-Qi LU
2
;
Xiao ZHANG
2
;
Hong-Wei WU
1
;
Li-Ying TANG
1
;
Zhu-Ju WANG
1
Author Information
1. Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China.
2. Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China College of Pharmacy,Henan University of Chinese Medicine Zhengzhou 450046,China.
- Publication Type:Journal Article
- Keywords:
Descurainiae Semen;
UPLC-MS/MS;
pharmacokinetic;
processing;
quercetin-3-O-β-D-glucose-7-O-β-D-gentiobioside;
sinapic acid
- MeSH:
Animals;
Chromatography, High Pressure Liquid;
Chromatography, Liquid;
Drugs, Chinese Herbal/pharmacokinetics*;
Rats;
Rats, Sprague-Dawley;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2019;44(22):4947-4952
- CountryChina
- Language:Chinese
-
Abstract:
An UPLC-MS/MS method simultaneously determining contents of quercetin-3-O-β-D-glucose-7-O-β-D-gentiobioside and sinapic acid in rats' plasma was firstly established and applied to study the effects of processing on pharmacokinetics of Descurainiae Semen's active constituents. Complantatoside A as internal standard,methanol used for protein precipitation,the method was validated according to the instructions of CFDA. Rats' plasma was collected after being oral administrated equal dosage of 60% ethanal extract of raw or processed Descurainiae Semen at different point of time,then the concentrations were determined to calculate pharmacokinetic parameters using DAS 3. 2. 6. And the parameters were analyzed using SPSS 23. 0,meantime the concentration-time curve was drawn.The results showed that processing had no effects on the pharmacokinetics of QGG,but could improve the absorption of sinapic acid and slow down the excretion.
