Mechanisms of Fuke Qianjin Capsules in treatment of pelvic inflammatory disease based on GC-MS metabolomics.
10.19540/j.cnki.cjcmm.20190804.301
- Author:
Bo-Hou XIA
1
;
Lu BAI
2
;
Ying-Shuai ZHANG
2
;
Su-Hui XIONG
1
;
Ya-Mei LI
1
;
Li-Mei LIN
1
;
Yun GONG
2
Author Information
1. Key Laboratory for Quality Evaluation of Bulk Herbs of Hunan Province,Hunan University of Chinese Medicine Changsha 410208,China.
2. Zhuzhou Qianjin Pharmaceutical Co.,Ltd. Zhuzhou 412003,China.
- Publication Type:Journal Article
- Keywords:
Fuke Qianjin Capsules;
GC-MS;
metabolomics;
pelvic inflammatory disease
- MeSH:
Animals;
Biomarkers;
Capsules;
Drugs, Chinese Herbal/therapeutic use*;
Female;
Gas Chromatography-Mass Spectrometry;
Humans;
Metabolomics;
Pelvic Inflammatory Disease/drug therapy*;
Rats
- From:
China Journal of Chinese Materia Medica
2019;44(22):4940-4946
- CountryChina
- Language:Chinese
-
Abstract:
Pelvic inflammatory disease( PID) rat model was induced by the mixture of Escherichia coli,Staphylococcus aureus,and Streptococcus hemolytic-β． Gas chromatography-mass spectrometry( GC-MS) based metabolic profiling method was combined with multivariate statistical analysis,such as PCA,PLS-DA and OPLS-DA to analyze endogenous small molecule metabolites in serum of rats after treatment of Fuke Qianjin Capsules. The results showed that Fuke Qianjin Capsules could significantly improve the inflammatory pathological characteristics and tissue damages in model rats. Based on the principle of VIP>1 and P<0. 05,a total of 6 different metabolic biomarkers were identified,including L-valine,L-isoleucine,L-threonine,butanedioic acid,serine and D-glucose,respectively.The contents of these six different metabolites were significantly reversed after administration. Further analysis of the metabolite pathways through KEGG database showed that Fuke Qianjin Capsules achieved the effect possibly through glycine,serine and threonine metabolism,aminoacyl-tRNA biosynthesis and valine,leucine and isoleucine biosynthesis. Therefore,this study came to the conclusion that Fuke Qianjin Capsules can be used in the treatment of mixed bacteria induced pelvic inflammatory disease possibly by regulating amino acid and its derivative metabolism.
