Analysis of polarity components of Angelicae Sinensis Radix and its metabolites in rats by HPLC-MS~n.
10.19540/j.cnki.cjcmm.20190522.503
- Author:
Lu WANG
1
;
Shan-Shan FAN
2
;
Feng XU
2
;
Guang-Xue LIU
2
;
Ming-Ying SHANG
2
;
Shao-Qing CAI
2
Author Information
1. Division of Pharmacognosy,School of Pharmaceutical Sciences,Peking University Beijing 100191,China Beijing Pharma and Biotech Center Beijing 100193,China.
2. Division of Pharmacognosy,School of Pharmaceutical Sciences,Peking University Beijing 100191,China.
- Publication Type:Journal Article
- Keywords:
Angelicae Sinensis Radix;
HPLC-ESI-IT-TOF-MS~n;
metabolites;
polarity components
- MeSH:
Animals;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
Glycosides;
Medicine, Chinese Traditional;
Rats;
Spectrometry, Mass, Electrospray Ionization
- From:
China Journal of Chinese Materia Medica
2019;44(22):4924-4931
- CountryChina
- Language:Chinese
-
Abstract:
This experiment aims to explore the metabolites of n-butanol and water soluble fraction of an ethanol extracts from Angelicae Sinensis Radix in rats. The chemical constituents of n-butanol and water extracts from Angelicae Sinensis Radix were identified by HPLC-DAD-ESI-IT-TOF-MS~n,and the in vivo metabolites of n-butanol and water extracts were analyzed. By analyzing n-butanol and water extracts from Angelicae Sinensis Radix,25 compounds were detected and identified,in which 11 phthalide glycosides were firstly reported. And 19 compounds were detected and identified in rat urine,including 2 prototype constituents and 17 metabolites,and the17 metabolites were new compounds. The method can identify the main constituents and metabolites of extracts from traditional Chinese medicine accurately and rapidly,and provide evidence for interpreting effective forms and pharmacodynamics substance( prototype,metabolites,or both) of Angelicae Sinensis Radix.
