Transcriptome analysis of venom gland and identification of functional genes for snake venom protein in Agkistrodon acutus.
10.19540/j.cnki.cjcmm.20190829.105
- Author:
Sheng-Xiang ZHANG
1
;
Yuan-Yuan SHI
1
;
Chun-Miao SHAN
1
;
Tao WANG
2
;
Zhen-Xing WANG
2
;
Sheng-Song WANG
3
;
Jia-Wen WU
4
Author Information
1. Anhui University of Chinese Medicine and Anhui Academy of Chinese Medicine Hefei 230012,China Key Laboratory of Xin'an Medicine,Ministry of Education,Anhui University of Chinese Medicine Hefei 230038,China.
2. Anhui Institute of Qi'men Snake Medicine Huangshang 245600,China.
3. Anhui University of Chinese Medicine and Anhui Academy of Chinese Medicine Hefei 230012,China Anhui Institute of Qi'men Snake Medicine Huangshang 245600,China.
4. Anhui University of Chinese Medicine and Anhui Academy of Chinese Medicine Hefei 230012,China Key Laboratory of Xin'an Medicine,Ministry of Education,Anhui University of Chinese Medicine Hefei 230038,China Synergetic Innovation Center of Anhui Authentic Chinese Medicine Quality Improvement Hefei 230012,China.
- Publication Type:Journal Article
- Keywords:
Agkistrodon acutus;
C-type lectin;
secretory phospholipase A_2;
transcriptome;
venom gland
- MeSH:
Agkistrodon/genetics*;
Animals;
Crotalid Venoms;
Gene Expression Profiling;
Snake Venoms/genetics*;
Snakes;
Transcriptome
- From:
China Journal of Chinese Materia Medica
2019;44(22):4820-4829
- CountryChina
- Language:Chinese
-
Abstract:
Agkistrodon acutus is a traditional Chinese herb medicine which has immunological regulation,anti-tumor,anti-inflammatory and analgesic effects,which is mainly used for the treatment of rheumatoid arthritis,ankylosing spondylitis,sjogren's syndrome and tumors. In order to excavate more important functional genes from A. acutus,the transcriptome of the venom gland was sequenced by the Illumina Hi Seq 4000,and 32 862 unigenes were assembled. Among them,26 589 unigenes were mapped to least one public database. 2 695 unigenes were annotated and assigned to 62 TF families,and 5 920 SSR loci were identified. The majority of mapped unigenes was from Protobothrops mucrosquamatus in the NR database,which revealed their closest homology. Three secretory phospholipase A_2 with different amino acid sequences showed similar spatial structures and all had well-conserved active sites. The 3 D structural models of C-type lectin showed conserved glycosylation binding sites( Asn45). This study will lay the foundation for the further study of the function of snake venom protein,and promoting the development and utilization of genome resources from A. acutus.
