Role of Eukaryotic Translation Elongation Factor 1 Family Members in the Tumorigenesis and Progression of Lung Adenocarcinoma.
10.3881/j.issn.1000-503X.15580
- Author:
Yue WU
1
;
Jiang-Feng LIU
2
;
Wan-Feng LIANG
1
;
Ye-Hong YANG
2
;
Gang HU
1
;
Jun-Tao YANG
2
Author Information
1. School of Statistics and Data Science,Nankai University,Tianjin 300071,China.
2. State Key Laboratory of Common Mechanism Research for Major Diseases,Institute of Basic Medical Sciences, CAMS and PUMC,Beijing 100005,China.
- Publication Type:Journal Article
- Keywords:
eukaryotic translation elongation factor 1;
immune;
lung adenocarcinoma;
phosphatidylinositol 3-kinase/protein kinase B signaling pathway
- MeSH:
Humans;
Peptide Elongation Factor 1/metabolism*;
Proteomics;
Proto-Oncogene Proteins c-akt/metabolism*;
Carcinogenesis;
Adenocarcinoma of Lung;
Lung Neoplasms;
RNA, Messenger/genetics*;
Phosphatidylinositol 3-Kinases;
Prognosis
- From:
Acta Academiae Medicinae Sinicae
2023;45(6):867-885
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role and mechanism of eukaryotic translation elongation factor 1(EEF1) family members (EEF1D,EEF1A1,and EEF1A2) in lung adenocarcinoma (LUAD) based on public databases.Methods We examined EEF1 member expression levels in human LUAD samples via The Cancer Genome Atlas in the UCSC Xena browser and the Clinical Proteomic Tumor Analysis Consortium.We analyzed the mRNA and protein levels of EEF1D,EEF1A1,and EEF1A2 and their correlations with pathological variables via the Mann-Whitney U test.The Kaplan-Meier curves were established to assess the prognostic values of EEF1D,EEF1A1,and EEF1A2.The single-sample gene set enrichment analysis algorithm was employed to explore the relationship between the expression levels of EEF1 members and tumor immune cell infiltration.Spearman and Pearson correlation analyses were performed to examine the relationship between the expression levels of EEF1 members and those of the genes in the phosphatidylinositol 3-kinase/protein kinase B signaling pathway.The immunohistochemical assay was employed to determine the expression levels of EEF1D,EEF1A1,and EEF1A2 in the LUAD tissue (n=75) and paracancer tissue (n=75) samples.Results The mRNA and protein levels of EEF1D,EEF1A1,and EEF1A2 showed significant differences between tumor and paracancer tissues (all P<0.001).The patients with high protein levels of EEF1A1 showed bad prognosis in terms of overall survival (P=0.039),and those with high protein levels of EEF1A2 showed good prognosis in terms of overall survival (P=0.012).The influence of the mRNA level of EEF1D on prognosis was associated with pathological characteristics.The expression levels of EEF1 members were significantly associated with the infiltration of various immune cells and the expression of key molecules in the phosphatidylinositol 3-kinase/protein kinase B signaling pathway.Conclusion EEF1D,EEF1A1,and EEF1A2 are associated with the progression of LUAD,serving as the candidate prognostic markers for LUAD.
