Inhibitory Effects and Mechanisms of Three Benzodiazepines on Helicobacter pylori.
10.3881/j.issn.1000-503X.15651
- Author:
Xu CHEN
1
;
Dong ZHANG
2
;
Li-Hua TANG
3
;
Li-Jun ZHONG
3
;
Juan ZHANG
3
;
Yu-Lan LI
4
Author Information
1. The First School of Clinical Medicine,Lanzhou University,Lanzhou 730000,China.
2. Department of Gastroenterology,Jiuquan City People's Hospital,Jiuquan,Gansu 735000,China.
3. Department of Microbiology,Jiuquan City People's Hospital,Jiuquan,Gansu 735000,China.
4. Department of Anesthesiology,The First Hospital of Lanzhou University,Lanzhou 730000,China.
- Publication Type:Journal Article
- Keywords:
antibacterial;
benzodiazepines
- MeSH:
Midazolam;
Helicobacter pylori;
Urease;
Clarithromycin/pharmacology*;
Benzodiazepines/pharmacology*;
Diazepam/pharmacology*;
Amoxicillin;
Water;
Anti-Bacterial Agents/pharmacology*
- From:
Acta Academiae Medicinae Sinicae
2023;45(5):783-788
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the inhibitory effects and mechanisms of benzodiazepines on Helicobacter pylori (Hp).Methods The Hp international standard strain ATCC43504 was treated with benzodiazepines diazepam,midazolam,and remimazolam,respectively.The treatments with amoxicillin and clarithromycin were taken as the positive controls,and that with water for injection as the negative control.The inhibition zone of each drug was measured by the disk diffusion method.The minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of each drug against Hp were determined.Hp suspension was configured and treated with diazepam and midazolam,respectively.The bacterial suspension without drug added was used as the control group.The concentration of K+ in each bacterial suspension was measured by an automatic biochemical analyzer before drug intervention(T0)and 1(T1),2(T2),3(T3),4(T4),5(T5),6(T6),and 7 h(T7)after intervention.Hp urease was extracted and treated with 1/2 MIC diazepam,1 MIC diazepam,2 MIC diazepam,1/2 MIC midazolam,1 MIC midazolam,2 MIC midazolam,1 mg/ml acetohydroxamic acid,and water for injection,respectively.The time required for the rise from pH 6.8 to pH 7.7 in each group was determined by the phenol red coloring method.Results The inhibition zones of diazepam,midazolam,remimazolam,amoxicillin,clarithromycin,and water for injection against Hp were 52.3,42.7,6.0,72.3,60.8,and 6.0 mm,respectively.Diazepam and midazolam showed the MIC of 12.5 μg/ml and 25.0 μg/ml and the MBC of 25 μg/ml and 50 μg/ml,respectively,to Hp.The concentrations of K+ in the diazepam,midazolam,and control groups increased during T1-T7 compared with those at T0(all P<0.01).The concentration of K+ in diazepam and midazolam groups during T1-T4 was higher than that in the control group(all P<0.01).The time of inhibiting urease activity in the 1/2 MIC diazepam,1 MIC diazepam,2 MIC diazepam,1/2 MIC midazolam,1 MIC midazolam,and 2 MIC midazolam groups was(39.86±5.11),(36.52±6.65),(38.58±4.83),(39.25±6.19),(36.36±4.61),and(35.81±6.18)min,respectively,which were shorter than that in the acetohydroxamic acid group(all P<0.01)and had no significance differences from that in the water for injection group(all P>0.05).Conclusion Diazepam and midazolam exerted inhibitory effects on Hp,which may be related to the cleavage of Hp cells rather than inhibiting urease.
