Exploration of cross-cultivar group characteristics of a new cultivar of <i>Prunus mume</i> 'Zhizhang Guhong Chongcui'.

Xiaotian Qin; Mengge Guo; Shaohua Qin; Ruidan Chen

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Exploration of cross-cultivar group characteristics of a new cultivar of Prunus mume 'Zhizhang Guhong Chongcui'.

Author: Xiaotian QIN ¹ ; Mengge GUO ¹ ; Shaohua QIN ² ; Ruidan CHEN ¹
Author Information

1. Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Beijing Laboratory of Urban and Rural Ecological Environment, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, National Engineering Research Center for Floriculture, Engineering Research Center of Landscape Environment of Ministry of Education, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, China.
2. Yuxi Institute of Mei, Sanmenxia 472200, Henan, China.
Publication Type:Journal Article
Keywords: Prunus mume; cross-cultivar group; flower color; real-time quantitative PCR; xylem color
MeSH: Animals; Anthocyanins; DNA Shuffling; Flowers/genetics*; Porifera; Prunus/genetics*; Glutamine/analogs & derivatives*; Plant Extracts
From: Chinese Journal of Biotechnology 2024;40(1):239-251
CountryChina
Language:Chinese
Abstract: 'Zhizhang Guhong Chongcui' is a new cultivar of Prunus mume with cross-cultivar group characteristics. It has typical characteristics of cinnabar purple cultivar group and green calyx cultivar group. It has green calyx, white flower, and light purple xylem, but the mechanism remains unclear. In order to clarify the causes of its cross-cultivar group traits, the color phenotype, anthocyanin content and the expression levels of genes related to anthocyanin synthesis pathway of 'Zhizhang Guhong Chongcui', 'Yuxi Zhusha' and 'Yuxi Bian Lü'e' were determined. It was found that the red degree of petals, sepals and fresh xylem in branches was positively correlated with the total anthocyanin content. MYBɑ1, MYB1, and bHLH3 were the key transcription factor genes that affected the redness of the three cultivars of flowers and xylem. The transcription factors further promoted the high expression of structural genes F3'H, DFR, ANS and UFGT, thereby promoting the production of red traits. Combined with phenotype, anthocyanin content and qRT-PCR results, it was speculated that the white color of petals of 'Zhizhang Guhong Chongcui' were derived from the high expression of FLS, F3'5'H, LAR and ANR genes in other branches of cyanidin synthesis pathway, and the low expression of GST gene. The green color of sepals might be originated from the relatively low expression of F3'H, DFR and ANS genes. The red color of xylem might be derived from the high expression of ANS and UFGT genes. This study made a preliminary explanation for the characteristics of the cross-cultivar group of 'Zhizhang Guhong Chongcui', and provided a reference for molecular breeding of flower color and xylem color of Prunus mume.