The role of iron-uptake factor PiuB in pathogenicity of soybean pathogen <i>Xanthomonas axonopodis</i> pv. <i>glycines</i>.

Ruyi SU; Luojia Jin; Jiangling XU; Huiya Geng; Xiao Chen; Siyi Lin; Wei Guo; Zhiyuan JI

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The role of iron-uptake factor PiuB in pathogenicity of soybean pathogen Xanthomonas axonopodis pv. glycines.

Author: Ruyi SU ¹ ; Luojia JIN ¹ ; Jiangling XU ¹ ; Huiya GENG ¹ ; Xiao CHEN ¹ ; Siyi LIN ¹ ; Wei GUO ¹ ; Zhiyuan JI ²
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1. College of Life Sciences, Zhejiang Normal University, Jinhua 321004, Zhejiang, China.
2. National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China.
Publication Type:Journal Article
Keywords: Fe3+; Xanthomonas axonopodis pv. glycines; iron-uptake factor PiuB; pathogenicity
MeSH: Iron; Glycine max; Virulence; Xanthomonas axonopodis/genetics*; Hydrogen Peroxide
From: Chinese Journal of Biotechnology 2024;40(1):177-189
CountryChina
Language:Chinese
Abstract: Iron is an essential element for living organisms that plays critical roles in the process of bacterial growth and metabolism. However, it remains to be elucidated whether piuB encoding iron-uptake factor is involved in iron uptake and pathogenicity of Xanthomonas axonopodis pv. glycines (Xag). To investigate the function of piuB, we firstly generated a piuB deletion mutant (ΔpiuB) by homologous recombination. Compared with the wild-type, the piuB mutant exhibited significantly reduced growth and virulence in host soybean. The mutant displayed markedly increased siderophore secretory volume, and its sensitivity to Fe³⁺, Cu²⁺, Zn²⁺ and Mn²⁺ was significantly enhanced. Additionally, the H₂O₂ resistance, exopolysaccharide yield, biofilm formation, and cell mobility of ΔpiuB were significantly diminished compared to that of the wild-type. The addition of exogenous Fe³⁺ cannot effectively restore the above characteristics of ΔpiuB. However, expressing piuB in trans rescued the properties lost by ΔpiuB to the levels in the wild-type. Taken together, our results demonstrated that PiuB is a potential factor for Xag to assimilate Fe³⁺, and is necessary for Xag to be pathogenic in host soybean.