Prokaryotic expression and biological activities of the hemolysin BL subunit of a pathogenic <i>Bacillus cereus</i> of cattle origin.

Yunjiao CHEN; Yunjiang HE; Qinglei MENG; Zhilin LIU; Xin ZHANG; Zelin JIA; Jiayu CUI; Xueli WANG

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Prokaryotic expression and biological activities of the hemolysin BL subunit of a pathogenic Bacillus cereus of cattle origin.

Author: Yunjiao CHEN ¹ ; Yunjiang HE ¹ ; Qinglei MENG ¹ ; Zhilin LIU ¹ ; Xin ZHANG ¹ ; Zelin JIA ¹ ; Jiayu CUI ¹ ; Xueli WANG ¹
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1. College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao 028042, Inner Mongolia, China.
Publication Type:Journal Article
Keywords: Bacillus cereus; cattle; hemolysin BL subunit; prokaryotic expression
MeSH: Cattle; Animals; Mice; Bacterial Proteins/metabolism*; Bacillus cereus/metabolism*; Hemolysin Proteins/metabolism*; Virulence Factors/metabolism*; Enterotoxins/metabolism*
From: Chinese Journal of Biotechnology 2023;39(12):4939-4949
CountryChina
Language:Chinese
Abstract: Bacillus cereus belongs to Gram-positive bacteria, which is widely distributed in nature and shows certain pathogenicity. Different B. cereus strains carry different subsets of virulence factors, which directly determine the difference in their pathogenicity. It is therefore important to study the distribution of virulence factors and the biological activity of specific toxins for precise prevention and control of B. cereus infection. In this study, the hemolysin BL triayl was expressed, purified, and characterized. The results showed that the bovine pathogenic B. cereus hemolysin BL could be expressed and purified in the prokaryotic expression system, and the bovine pathogenic B. cereus hemolysin BL showed hemolysis, cytotoxicity, good immunogenicity and certain immune protection in mice. In this study, the recombinant expression of hemolysin BL triayl was achieved, and the biological activity of hemolysin BL of bovine pathogenic ceroid spore was investigated. This study may facilitate further investigating the pathogenic mechanism of B. cereus hemolysin BL and developing a detection method for bovine pathogenic B. cereus disease.