Evaluation of the humoral immunity in mice induced by foot-and-mouth disease virus-like particles-ZIF-8 complexes with different sizes.

Jiajun LI; Jun Wang; Yun Zhang; Zhidong Teng; Hu Dong; Huichen Guo; Shiqi Sun

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Evaluation of the humoral immunity in mice induced by foot-and-mouth disease virus-like particles-ZIF-8 complexes with different sizes.

Author: Jiajun LI ¹ ; Jun WANG ¹ ; Yun ZHANG ¹ ; Zhidong TENG ¹ ; Hu DONG ¹ ; Huichen GUO ¹ ; Shiqi SUN ¹
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1. State Key Laboratory for Animal Disease Control and Prevention, National Foot-and-mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, Gansu, China.
Publication Type:Journal Article
Keywords: adjuvants; foot-and-mouth disease virus-like particles (FMDV VLPs); size effect; zeolitic imidazolate framework-8 (ZIF-8)
MeSH: Animals; Mice; Foot-and-Mouth Disease/prevention & control*; Foot-and-Mouth Disease Virus; Antibodies, Neutralizing; Immunity, Humoral; Immunization; Vaccines, Virus-Like Particle; Antibodies, Viral; Viral Vaccines
From: Chinese Journal of Biotechnology 2023;39(12):4837-4848
CountryChina
Language:Chinese
Abstract: To further enhance the immune effect of the foot-and-mouth disease (FMD) virus-like particles (VLPs) vaccine, this study prepared FMDV VLPs-zeolitic imidazolate (framework-8, ZIF-8) complexes with different particle sizes. We used a biomimetic mineralization method with Zn²⁺ and 2-methylimidazole in different concentration ratios to investigate the effect of size on the immunization effect. The results showed that FMDV VLPs-ZIF-8 with three different sizes were successfully prepared, with an approximate size of 70 nm, 100 nm, and 1 000 nm, respectively. Cytotoxicity and animal toxicity tests showed that all three complexes exhibited excellent biological safety. Immunization tests in mice showed that all three complexes enhanced the titers of neutralizing and specific antibodies, and their immune effects improved as the size of the complexes decreased. This study showed that ZIF-8 encapsulation of FMDV VLPs significantly enhanced their immunogenic effect in a size-dependent manner.