Visualization method of type Ⅳ pili and its application in the study of pili function.

Author: Yan LUO ¹ ; Jingchao ZHANG ²
Author Information

1. Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, China.
2. Medical Genetics Center, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China.
Publication Type:Journal Article
Keywords: function; type Ⅳ pili; visualization
MeSH: Fimbriae, Bacterial/metabolism*; Bacterial Proteins/genetics*; Bacterial Physiological Phenomena; Bacterial Adhesion/physiology*
From: Chinese Journal of Biotechnology 2023;39(11):4534-4549
CountryChina
Language:Chinese
Abstract: As an important protein structure on the surface of bacteria, type Ⅳ pili (TFP) is the sensing and moving organ of bacteria. It plays a variety of roles in bacterial physiology, cell adhesion, host cell invasion, DNA uptake, protein secretion, biofilm formation, cell movement and electron transmission. With the rapid development of research methods, technical equipment and pili visualization tools, increasing number of studies have revealed various functions of pili in cellular activities, which greatly facilitated the microbial single cell research. This review focuses on the pili visualization method and its application in the functional research of TFP, providing ideas for the research and application of TFP in biology, medicine and ecology.