Recent advances in poly phosphate kinase (PPK) and the construction of PPK-mediated ATP regeneration system.

Author: Feng CHENG ¹ ; Huan LI ¹ ; Kexin LI ¹ ; Haiyun LIU ¹ ; Qi SHEN ¹ ; Yaping XUE ¹ ; Yuguo ZHENG ¹
Author Information

1. Key Laboratory of Bioorganic Synthesis of Zhejiang Province, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, China.
Publication Type:Journal Article
Keywords: ATP regeneration; biosynthesis; poly phosphate kinase; protein engineering
MeSH: Adenosine Triphosphate/metabolism*; Adenosine Monophosphate; Polyphosphates/metabolism*; Catalysis; Regeneration
From: Chinese Journal of Biotechnology 2023;39(11):4413-4427
CountryChina
Language:Chinese
Abstract: Adenosine triphosphate (ATP) regeneration systems are essential for efficient biocatalytic phosphoryl transfer reactions. Polyphosphate kinase (PPK) is a versatile enzyme that can transfer phosphate groups among adenosine monophosphate (AMP), adenosine diphosphate (ADP), ATP, and polyphosphate (Poly P). Utilization of PPK is an attractive solution to address the problem of ATP regeneration due to its ability to use a variety of inexpensive and stable Poly P salts as phosphate group donors. This review comprehensively summarizes the structural characteristics and catalytic mechanisms of different types of PPKs, as well as the variations in enzyme activity, catalytic efficiency, stability, and coenzyme preference observed in PPKs from different sources. Moreover, recent advances in PPK-mediated ATP regeneration systems and protein engineering of wild-type PPK are summarized.