Engineering and application of Komagataella phaffii as a cell factory.
- Author:
Yufei LIU
1
;
Ying CAO
1
;
Liye CHANG
1
;
Conghui SHAN
1
;
Kun XU
1
Author Information
- Publication Type:Journal Article
- Keywords: Komagataella phaffii; cell factory; gene editing; metabolic engineering; synthetic biology
- MeSH: Saccharomycetales/genetics*; Genetic Techniques
- From: Chinese Journal of Biotechnology 2023;39(11):4376-4396
- CountryChina
- Language:Chinese
- Abstract: Nowadays, engineered Komagataella phaffii plays an important role in the biosynthesis of small molecule metabolites and protein products, showing great potential and value in industrial productions. With the development and application of new editing tools such as CRISPR/Cas9, it has become possible to engineer K. phaffii into a cell factory with high polygenic efficiency. Here, the genetic manipulation techniques and objectives for engineering K. phaffii are first summarized. Secondly, the applications of engineered K. phaffii as a cell factory are introduced. Meanwhile, the advantages as well as disadvantages of using engineered K. phaffii as a cell factory are discussed and future engineering directions are prospected. This review aims to provide a reference for further engineering K. phaffii cell factory, which is supposed to facilitate its application in bioindustry.
