Advances in the application of yeast surface display technology.

Lili Zhao; Bingkai SU; Shushu DU; Wenting Ding; Rongzeng Liu

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Advances in the application of yeast surface display technology.

Author: Lili ZHAO ¹ ; Bingkai SU ¹ ; Shushu DU ¹ ; Wenting DING ¹ ; Rongzeng LIU ¹
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1. Department of Immunology, School of Basic Medical Sciences, Henan University of Science and Technology, Luoyang 471023, Henan, China.
Publication Type:Journal Article
Keywords: antibody engineering; enzyme engineering; library construction; oral vaccine; protein engineering; yeast surface display technology
MeSH: Saccharomyces cerevisiae/metabolism*; Protein Engineering; Biotechnology; Antibodies/metabolism*; Amino Acid Sequence
From: Chinese Journal of Biotechnology 2023;39(11):4358-4375
CountryChina
Language:Chinese
Abstract: Yeast surface display (YSD) is a technology that fuses the exogenous target protein gene sequence with a specific vector gene sequence, followed by introduction into yeast cells. Subsequently, the target protein is expressed and localized on the yeast cell surface by using the intracellular protein transport mechanism of yeast cells, whereas the most widely used YSD system is the α-agglutinin expression system. Yeast cells possess the eukaryotic post-translational modification mechanism, which helps the target protein fold correctly. This mechanism could be used to display various eukaryotic proteins, including antibodies, receptors, enzymes, and antigenic peptides. YSD has become a powerful protein engineering tool in biotechnology and biomedicine, and has been used to improve a broad range of protein properties including affinity, specificity, enzymatic function, and stability. This review summarized recent advances in the application of YSD technology from the aspects of library construction and screening, antibody engineering, protein engineering, enzyme engineering and vaccine development.