Optimization of retinin expression and the application with wax emulsion in nanocoatings.
- Author:
Yuqing LIU
1
;
Yuanyuan XIA
1
;
Wei SHEN
1
;
Haiquan YANG
1
;
Xianzhong CHEN
1
Author Information
- Publication Type:Journal Article
- Keywords: anti-reflective; expression optimization; nanocoatings; retinin
- MeSH: Animals; Cricetinae; CHO Cells; Emulsions; Cricetulus; Drosophila; Eye Proteins; Drosophila Proteins
- From: Chinese Journal of Biotechnology 2023;39(10):4258-4274
- CountryChina
- Language:Chinese
- Abstract: Anti-reflective nanocoatings that mimic the eyes of fruit flies are biodegradable materials with great market potential for a variety of optical devices that require anti-reflective properties. Microbial expression of retinin provides a new idea for the preparation of nanocoatings under mild conditions compared to physicochemical methods. However, the current expression level of retinin, the key to anti-reflective coating, is low and difficult to meet mass production. In this study, we analyzed and screened the best expression hosts for Drosophila-derived retinin protein, and optimized its expression. Chinese hamster ovary (CHO) cells were identified as the efficient expression host of retinin, and purified retinin protein was obtained. At the same time, the preparation method of lanolin nanoemulsion was explored, and the best anti-reflective ability of the nano-coating was determined when the ratio of specific concentration of retinin protein and wax emulsion was 16:4, the pH of the nano-coating formation system was 7.0, and the temperature was 30 ℃. The enhanced antireflective ability and reduced production cost of artificial antireflective nanocoatings by determining the composition of nanocoatings and optimizing the concentration, pH and temperature of system components may facilitate future application of artificial green degradable antireflective coatings.
