Production and antigenicity analysis of a recombinant insulinoma associated protein-2 in HEK293 cells.
- Author:
Jingwen QIAN
1
;
Pengbo WANG
2
;
Yuanxing ZHANG
2
;
Qin LIU
2
Author Information
- Publication Type:Journal Article
- Keywords: autoimmunity; insulinoma-associated protein-2; purification of membrane protein; type 1 diabetic mellitus
- MeSH: Humans; Animals; Cattle; HEK293 Cells; Insulinoma; Diabetes Mellitus, Type 1/genetics*; Recombinant Proteins; Membrane Proteins; Pancreatic Neoplasms
- From: Chinese Journal of Biotechnology 2023;39(10):4246-4257
- CountryChina
- Language:Chinese
- Abstract: Insulinoma-associated protein-2 (IA-2) is a transmembrane glycoprotein belonging to the tyrosine phosphatase-like protein family as well as an important autoantigen in the diagnosis of type 1 diabetes. IA-2 products have been marketed in Europe and the United States. At present, commercially available IA-2 antigens are either the recombinant IA-2ic domain or the IA-2 naturally extracted from bovine islets. However, the recombinant IA-2 antigen displays weak positive in clinic practice, which often results in occasional detection failures, thus cannot completely replace the naturally extracted IA-2 antigen. In this study, an HEK293 expression system was used to explore the production of recombinant IA-2. An IA-2 transmembrane fragment (IA-2 TMF) located at amino acid position 449-979, also known as the natural membrane protein form of IA-2, was produced in HEK293 through transfection, and both the expression conditions and dissolution conditions of the membrane protein were also optimized. The purified membrane protein yield was 0.78 mg/L cell culture. Subsequently, the antigen activity of IA-2 TMF was compared with RSR rhIA-2 through enzyme linked immunosorbent assay. The serum of 77 type 1 diabetes patients and 32 healthy volunteers were detected. Receiver operating characteristic curve (ROC) curve was used to characterize the sensitivity and specificity of the test results. The results showed that the sensitivity of IA-2 TMF was 71.4% (55/77), while the sensitivity of RSR rhIA-2 was 63.6% (49/77), and the specificity of both antigens were all 100%. There was no significant difference in specificity between the two antigens, but the sensitivity of IA-2 TMF was appreciably better than that of the imported gold standard RSR rhIA-2 antigen. In conclusion, the recombinant IA-2 TMF produced in HEK293 cells can be used as a raw material to develop in vitro diagnostic reagents for type 1 diabetes.
