Application of single base editing technique in pig genetic improvement: a review.

Wudi Zhao; Guobin Huang; Xiangxing Zhu; Yanzhen BI; Dongsheng Tang

Return

Application of single base editing technique in pig genetic improvement: a review.

Author: Wudi ZHAO ¹ ; Guobin HUANG ¹ ; Xiangxing ZHU ² ; Yanzhen BI ³ ; Dongsheng TANG ¹
Author Information

1. Guangdong Key Laboratory of Animal Molecular Design and Precise Breeding, School of Life Science and Engineering, Foshan University, Foshan 528225, Guangdong, China.
2. Guangdong Research Center of Gene Editing Engineering Technology, School of Medicine, Foshan University, Foshan 528225, Guangdong, China.
3. Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Institute of Animal Husbandry and Veterinary Science, Hubei Academy of Agricultural Sciences, Wuhan 430072, Hubei, China.
Publication Type:Journal Article
Keywords: CRISPR/Cas9; gene editing; genetic improvement; pig; single base editing
MeSH: Animals; Swine/genetics*; Gene Editing; CRISPR-Cas Systems/genetics*; DNA Breaks, Double-Stranded
From: Chinese Journal of Biotechnology 2023;39(10):3936-3947
CountryChina
Language:Chinese
Abstract: Traditional pig breeding has a long cycle and high cost, and there is an urgent need to use new technologies to revitalize the pig breeding industry. The recently emerged CRISPR/Cas9 genome editing technique shows great potential in pig genetic improvement, and has since become a research hotspot. Base editor is a new base editing technology developed based on the CRISPR/Cas9 system, which can achieve targeted mutation of a single base. CRISPR/Cas9 technology is easy to operate and simple to design, but it can lead to DNA double strand breaks, unstable gene structures, and random insertion and deletion of genes, which greatly restricts the application of this technique. Different from CRISPR/Cas9 technique, the single base editing technique does not produce double strand breaks. Therefore, it has higher accuracy and safety for genome editing, and is expected to advance the pig genetic breeding applications. This review summarized the working principle and shortcomings of CRISPR/Cas9 technique, the development and advantages of single base editing, the principles and application characteristics of different base editors and their applications in pig genetic improvement, with the aim to facilitate genome editing-assisted genetic breeding of pig.