A novel method for detecting circulating tumor cells immunity based on micro-nano technique.

Shuting LI; Shufen Jiao; Yu LI; Yujuan WU; Rongyun Zhai; Zhe Wang; Jing Cheng; Weiying Zhang; Yali Ben

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A novel method for detecting circulating tumor cells immunity based on micro-nano technique.

Author: Shuting LI ¹ ; Shufen JIAO ² ; Yu LI ³ ; Yujuan WU ¹ ; Rongyun ZHAI ¹ ; Zhe WANG ¹ ; Jing CHENG ⁴ ; Weiying ZHANG ⁵ ; Yali BEN ¹
Author Information

1. School of Medicine, Jianghan University, Wuhan 430056, Hubei, China.
2. Department of Psychiatry, Wuhan Mental Health Center, Wuhan 430012, Hubei, China.
3. Huangshi Central Hospital, Huangshi 435002, Hubei, China.
4. Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei, China.
5. College of Life Science, Jianghan University, Wuhan 430056, Hubei, China.
Publication Type:Journal Article
Keywords: biosignal amplification; carboxylated multi-walled carbon nanotubes (MWCNTs-COOH); circulating tumor cells; colorectal cancer; immunesensor
MeSH: Humans; Nanotubes, Carbon/chemistry*; Neoplastic Cells, Circulating/pathology*; Biosensing Techniques; Immunoassay/methods*; Antibodies; Colorectal Neoplasms/diagnosis*; Electrochemical Techniques/methods*; Gold/chemistry*
From: Chinese Journal of Biotechnology 2023;39(9):3849-3862
CountryChina
Language:Chinese
Abstract: This study was to develop a new method for detecting circulating tumor cells (CTCs) with high sensitivity and specificity, therefore to detect the colorectal cancer as early as possible for improving the detection rate of the disease. To this end, we prepared some micro-column structure microchips modified with graphite oxide-streptavidin (GO-SA) on the surface of microchips, further coupled with a broad-spectrum primary antibody (antibody1, Ab₁), anti-epithelial cell adhesion molecule (anti-EpCAM) monoclonal antibody to capture CTCs. Besides, carboxylated multi-walled carbon nanotubes (MWCNTs-COOH) were coupled with colorectal cancer related antibody as specific antibody 2 (Ab₂) to prepare complex. The sandwich structure consisting of Ab₁-CTCs-Ab₂ was constructed by the microchip for capturing CTCs. And the electrochemical workstation was used to detect and verify its high sensitivity and specificity. Results showed that the combination of immunosensor and micro-nano technology has greatly improved the detection sensitivity and specificity of the immunosensor. And we also verified the feasibility of the immunosensor for clinical blood sample detection, and successfully recognitized detection and quantization of CTCs in peripheral blood of colorectal cancer patients by this immunosensor. In conclusion, the super sandwich immunosensor based on micro-nano technology provides a new way for the detection of CTCs, which has potential application value in clinical diagnosis and real-time monitoring of disease.