Application of deep mutational scanning technology in protein research.

Yifan LI; Yi Wang; Kaili Zhang; Shuai LI

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Application of deep mutational scanning technology in protein research.

Author: Yifan LI ¹ ; Yi WANG ¹ ; Kaili ZHANG ¹ ; Shuai LI ¹
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1. Key Laboratory of Breast Cancer Prevention and Therapy, Ministry of Education, Tianjin Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Department of Breast Cancer Pathology and Research Laboratory, Tianjin Medical University Cancer Institute & Hospital, National Clinical Research Center for Cancer, Tianjin 300060, China.
Publication Type:Journal Article
Keywords: deep mutational scanning; high-throughput sequencing; library construction; mammalian cells
MeSH: Animals; Mutation; Proteins/chemistry*; Protein Engineering; High-Throughput Nucleotide Sequencing/methods*; Mammals/genetics*
From: Chinese Journal of Biotechnology 2023;39(9):3710-3723
CountryChina
Language:Chinese
Abstract: As central players in cellular structure and function, proteins have long been central themes in life science research. Analyzing the impact of protein sequence variation on its structure and function is one of the important means to study proteins. In recent years, a technology called deep mutational scanning (DMS) has been widely used in the field of protein research. It introduces thousands of mutations in parallel in specific regions of proteins through high-abundance DNA libraries. After screening, high-throughput sequencing is employed to score each mutation, revealing sequence-function correlations. Due to its high-throughput, fast and easy, and labor-saving features, DMS has become an important method for protein function research and protein engineering. This review briefly summarizes the principle of DMS technology, highlighting its applications in mammalian cells. Moreover, this review analyzes the current technical bottlenecks, aiming to facilitate relevant research.