Single chain antibody fragment display systems: a review.

Yao Chen; Xingfu Shu; Yu Zhao; Bowen Zhang; Zhongren MA; Haixia Zhang

Return

Single chain antibody fragment display systems: a review.

Author: Yao CHEN ¹ ; Xingfu SHU ¹ ; Yu ZHAO ¹ ; Bowen ZHANG ¹ ; Zhongren MA ¹ ; Haixia ZHANG ¹
Author Information

1. National Ethnic Affairs Commission Key Laboratory of Bioengineering and Technology, Biomedical Research Center, Northwest Minzu University, Lanzhou 730030, Gansu, China.
Publication Type:Journal Article
Keywords: genetically engineered antibody; phage display system; ribosome display system; single chain antibody fragment
MeSH: Animals; Immunoglobulin Variable Region/genetics*; Immunoglobulin Fragments/metabolism*; Single-Chain Antibodies/metabolism*; Peptide Library; Mammals/genetics*
From: Chinese Journal of Biotechnology 2023;39(9):3681-3694
CountryChina
Language:Chinese
Abstract: Single chain antibody fragment (scFv) is a small molecule composed of a variable region of heavy chain (VH) and a variable region of light chain (VL) of an antibody, and these two chains are connected by a flexible short peptide. scFv is the smallest functional fragment with complete antigen-binding activity, which contains both the antibody-recognizing site and the antigen-binding site. Compared with other antibodies, scFv has the advantages of small molecular weight, strong penetration, low immunogenicity, and easy expression. Currently, the most commonly used display systems for scFv mainly include the phage display system, ribosome display system, mRNA display system, yeast cell surface display system and mammalian cell display system. In recent years, with the development of scFv in the field of medicine, biology, and food safety, they have also attracted much attention in the sectors of biosynthesis and applied research. This review summarizes the advances of scFv display systems in recent years in order to facilitate scFv screening and application.