Phage antibody library technology in tumor therapy: a review.

Xiaoyang Chen; Ruiheng AN; Ju Huang; Youfeng Liang; Wenjing Zhang; Mingxuan Hao; Rui Guo; Xiaoning LI; Yongchao LI; Lu Ying; Zhao Yang

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Phage antibody library technology in tumor therapy: a review.

Author: Xiaoyang CHEN ¹ ; Ruiheng AN ¹ ; Ju HUANG ¹ ; Youfeng LIANG ¹ ; Wenjing ZHANG ¹ ; Mingxuan HAO ¹ ; Rui GUO ¹ ; Xiaoning LI ¹ ; Yongchao LI ² ; Lu YING ² ; Zhao YANG ¹
Author Information

1. Innovation Center of Molecular Diagnostics, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
2. Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production and Construction Corps, College of Life Science and Technology, Tarim University, Alar 843300, Xinjiang, China.
Publication Type:Journal Article
Keywords: markers; monoclonal antibody; phage antibody library technology; targeted therapy; tumor
MeSH: Humans; Bacteriophages/genetics*; Immunoglobulin Variable Region/genetics*; Gene Library; Antibodies, Monoclonal/therapeutic use*; Immunotherapy; Peptide Library
From: Chinese Journal of Biotechnology 2023;39(9):3644-3669
CountryChina
Language:Chinese
Abstract: Tumor is a serious threat to human health. At present, surgical resection, chemoradiotherapy, targeted therapy and immunotherapy are the main therapeutic strategies. Monoclonal antibody has gradually become an indispensable drug type in the clinical treatment of cancer due to its high efficiency and low toxicity. Phage antibody library technology (PALT) is a novel monoclonal antibody preparation technique. The recombinant immunoglobulin variable region of heavy chain (VH)/variable region of light chain (VL) gene is integrated into the phage vector, and the antibody is expressed on the phage surface in the form of fusion protein to obtain a diverse antibody library. Through the process of adsorption-elution-amplification, the antibody library can be screened to obtain the antibody molecule with specific binding antigen as well as its gene sequence. PALT has the advantages of short antibody production cycle, strong plasticity of antibody structure, large antibody yield, high diversity and direct production of humanized antibodies. It has been used in screening tumor markers and preparation of antibody drugs for breast cancer, gastric cancer, lung cancer and liver cancer. This article reviews the recent progress and the application of PALT in tumor therapy.